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A Flexible System Of Enzymatic Analysis

Author: Oliver Lowry
Publisher: Elsevier
ISBN: 0323156711
Size: 73.76 MB
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A Flexible System of Enzymatic Analysis is a multipurpose manual of laboratory methods that offers a systematic scheme for the analysis of biological materials from the level of the wholeorgan down to the single cell and beyond. It intends to guide the development of methods, the refinement of old ones, and the adaptation in general of methods to almost any scale of sensitivity. This manual is organized into three parts: a general section, one on quantitative histochemistry, and an appendix containing information that may be useful to have at the bench. The general section is comprised of nine chapters that focus on properties of the pyridine nucleotides, kinetics, and glassware, as well as on improvement, modification, adaptation, trouble shooting, and development of methods. This part also describes the preparation of tissues for analysis, the enzymatic cycling methods, and a compendium of 36 metabolite assays. The quantitative histochemistry section is comprised of four chapters that include information on the preparation of frozen-dried material and dissection of samples for analysis; the fishpole balance for weighing samples; and the generalities of analysis with emphasis on the ""oil well technique."" This book will be useful to novices as well as experts who are familiar with other analytical styles.

Enzymatic Analysis

Author: Janet V. Passonneau
Publisher: Springer Science & Business Media
ISBN: 1603274073
Size: 29.50 MB
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Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

Handbook Of Phycological Methods

Author: Phycological Society of America
Publisher: Cambridge University Press
ISBN: 9780521200493
Size: 30.45 MB
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This 1973 book was the first comprehensive collection of techniques for culturing algae. Twenty-eight clearly written chapters by 30 outstanding algologists utilize the work of more than 600 investigators. On its initial publication, the book became a standard reference in the field.

Transmethylations And The Central Nervous System

Author: V. M. Andreoli
Publisher: Springer Science & Business Media
ISBN: 3642885160
Size: 34.72 MB
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V M. ANDREOLl, A. AGNOLI, and C. FAZIO The transfer of a methyl group from a donor to an acceptor compound is a fundamental biochemie al process long since known to biologists; the process is involved, for instance, in the metabolism of porphyrins, nuc1eic acids, and fatty acids. Only recently, however, did transmethylation processes reveal their decisive role in CNS biochemistry - namely with the discovery that such processes are linked with the biogenie amines that have been conc1usively identified as the chemical mediators of neu ronal transmission and, more broadly, of behavior. The first suggestion that transmethylation processes might be involved in the origina tion of certain mental diseases came from Harley-Mason (1952), who noticed that many of the hallucinogenic substances known at that time contained methyl radicals, and par ticularly that mescaline represented the product of O-methylation of dopamine in posi tions 3, 4, and 5. This hypothesis was put forward when the O-methylation of catechol amines by catechol-O-methyltransferases had not yet been described. Harley-Mason further proposed that in the living organism the process of O-methyla tion might follow a "deviant" metabolie pathway, and that the accumulation of abnor mal methylated metabolites endowed with hallucinogenic properties might be responsi ble for the implementation of some mental diseases, notably schizophrenia. He also called attention to a substance, 3,4-dimethoxyphenylethylamine (3,4-DMPEA) as a possible psychotogenic moleeule, on the strength of its capacity for producing cata tonia in experimental animals.

Experimental And Clinical Hepatology

Author: C.E. Broelsch
Publisher: Springer Science & Business Media
ISBN: 940094151X
Size: 38.43 MB
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This is the fifth symposium on Experimental and Clinical Hepatology to be convened by the organisers, and, in common with the previous four, reflects the importance placed upon continuing discussion by clinical investigators as well as basic physiological and pharmacological researchers. Previous meetings which were held at the Surgical Department of the Philipps University in Marburg were dedicated to problems of direct clinical application, such as haemodynamics of portal hypertension, surgical approach to hepatic tumours, liver regeneration and temporary hepatic support. Clinical investigation was a subject which frequently came under discussion and some of these investigations have since lead to direct improvements in clinical therapy and many patients have benefited as a result. This time, the convocation was called to the Medizinische Hochschule Hannover where remarkable advances in clinical liver trans plantation have been the most striking event in clinical hepatology. The concensus of the meeting was that while significant clinical progress has been made, a variety of basic problems remained unsolved which will require further investigation. Special attention has, therefore, been given to topics such as acute liver damage, particu larly within the transplanted liver, to chronic active hepatitis and its spontaneous prognosis, to isolated hepatocytes and their effect on functional support of the liver, to mechanisms of liver circulation which may be altered completely within the transplant and to liver perfusion which is still considered unsatisfactory for subsequent transplantation.

Advanced Techniques In Soil Microbiology

Author: Ajit Varma
Publisher: Springer Science & Business Media
ISBN: 3540708650
Size: 33.84 MB
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This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.

Clinical Light Damage To The Eye

Author: David Miller
Publisher: Springer Science & Business Media
ISBN: 1461247047
Size: 40.79 MB
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To my mind, the superoxide radical discovered by Linus Pauling more than 50 years ago is about to become a major issue in Ameri can medicine. Uncannily, Pauling's early focus on vitamin C has pointed the way to the whole catalogue of free-radical scavengers, which we in medicine will be using in the coming decade. In ophthalmology, the basic scientists have been talking about the role of free-radical induction by light for some time. They have accumulated an increasing amount of evidence supporting the idea that prolonged light exposure contributes to cataract development and retinal degeneration. Through Clinical Light Damage to the Eye, we hope to bring this message to the practicing ophthalmolo gist. Because Dr. Pauling's work bears so strongly on the key issue of free-radical damage, and because of my own great respect for him as a scientist and a man of rare courage, I invited Dr. Pauling to write the foreword to Clinical Light Damage to the Eye, which follows.

Considerations In Contact Lens Use Under Adverse Conditions

Author: National Research Council
Publisher: National Academies Press
ISBN: 0309044383
Size: 38.42 MB
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This book summarizes current understanding of the scientific, clinical, and technical issues surrounding the use of contact lenses. It discusses the special occupational conditions experienced by military personnel, particularly in extreme environments, that give rise to the question of whether or not to use contact lenses. Experts in optometry, ophthalmology, visual psychophysics, and engineering describe recent developments in design and use; and representatives of the military services provide examples of actual situations in aerospace settings. Considerations in Contact Lens Use Under Adverse Conditions will be of particular interest to those involved in the design of contact lenses and those responsible for occupational safety and health matters in the private sector.

Enzyme Kinetics Catalysis And Control

Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0123809258
Size: 14.67 MB
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Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions