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A Laboratory Guide To The Mammalian Embryo

Author: David K. Gardner
Publisher: Oxford University Press
ISBN: 9780195142266
Size: 68.72 MB
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Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.

Single Cell Diagnostics

Author: Alan Thornhill
Publisher: Springer Science & Business Media
ISBN: 159745298X
Size: 39.32 MB
Format: PDF, Kindle
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This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.

Textbook Of Assisted Reproductive Technologies

Author: David. K Gardner
Publisher: CRC Press
ISBN: 0203092937
Size: 52.44 MB
Format: PDF, ePub
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Textbook of Assisted Reproductive Technologies is a truly comprehensive manual for the whole team at the IVF clinic. Information is presented in a highly visual manner, allowing both methods and protocols to be consulted easily. The text provides clinical and scientific teams with the A to Zs of setting up an embryology laboratory, gives research fellows insight into technical developments, and supplies seasoned professionals with a review of the latest techniques and advances. New to the Third Edition: fully revised and expanded chapters, with new information on: single embryo transfer artificial gametes pharmacogenetics

Textbook Of Assisted Reproductive Techniques Fourth Edition

Author: David K. Gardner
Publisher: CRC Press
ISBN: 1841849707
Size: 21.57 MB
Format: PDF, Kindle
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Textbook of Assisted Reproductive Techniques has become a classic comprehensive reference for the whole team at the IVF clinic. The fourth edition comes more conveniently as a set of two separate volumes, one for laboratory aspects and the other for clinical applications. The text has been extensively revised, with the addition of several important new contributions on laboratory aspects including developing techniques such as PICSI, IMSI, and time-lapse imaging. The second volume focuses on clinical applications and includes new chapters on lifestyle factors, tailored ovarian stimulation, frozen-thawed embryo transfer, viral disease, and religious perspectives. As before, methods, protocols, and techniques of choice are presented by eminent international experts. The two volume set includes: ■ Volume One - Laboratory Perspectives ■ Volume Two - Clinical Perspectives

Transgenic Animal Technology

Author: Carl A. Pinkert
Publisher: Newnes
ISBN: 0124095364
Size: 43.42 MB
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Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Guide To Techniques In Mouse Development

Author: Paul M. Wassarman
Publisher: Academic Pr
ISBN: 9780121821265
Size: 19.26 MB
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This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ cells and embryos; Fertilization; Gene expression: messenger RNA, reporter genes, proteins, methylation; Gene identification; Nuclear transplantation; Transgenic animals: pronuclear injection, embryonic stem cells, and gene targeting; Lineage analysis. Key Features * Contains the most up-to-date methodology as applied to mouse development * Focuses on molecular approaches to experimental mouse development * The first comprehensive source for molecular biological methods in mouse development * Many of the methods have never appeared before

Mammalian And Avian Transgenesis New Approaches

Author: Shirley Pease
Publisher: Springer Science & Business Media
ISBN: 9783540284895
Size: 50.65 MB
Format: PDF
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Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.