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A Practical Guide To Membrane Protein Purification

Author: Gebhard von Jagow
Publisher: Elsevier
ISBN: 0080571727
Size: 13.76 MB
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A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Provides general guidelines and strategies for isolation of membrane proteins Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs Gives special emphasis to new native and denaturing electrophoresis techniques Explains modifications of techniques used for water-soluble proteins

Barrel Channel Proteins As Tools In Nanotechnology

Author: Marco Fioroni
Publisher: Springer Science & Business Media
ISBN: 940077429X
Size: 74.12 MB
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ß-barrel outer membrane channel proteins (OMP) are useful as robust and flexible models or components in nanotechnology. Over the last decade biotechnological techniques allowed to expand the natural characteristics of OMPs by modifying their geometry and properties. The present book is oriented towards a broad group of readers including graduate students and advanced researchers. It gives a general introduction to the field of OMP based nano-component development as well as the state of the art of the involved research. On the example of the E. coli FhuA the transformation of an OMP into a tailored nano-channel will be outlined. An exhaustive description of the scientific strategy, including protein selection, analytical methods and “in-silico” tools to support the planning of protein modifications for a targeted application, consideration on the production of a custom made OMP, and an overview on technological applications including membrane/polymersome technology, will be provided.

E Coli Plasmid Vectors

Author: Nicola Casali
Publisher: Springer Science & Business Media
ISBN: 9781592594092
Size: 31.84 MB
Format: PDF, Kindle
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The authors present a comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, 1 vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids and the use of reporter genes, are also described.

Hplc Of Biological Macro Molecules Revised And Expanded

Author: Karen M. Gooding
Publisher: CRC Press
ISBN: 0824755057
Size: 44.10 MB
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Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

Molekular Und Zellbiologische Grundlagen

Author: Detlev Ganten
Publisher: Springer-Verlag
ISBN: 3662088223
Size: 75.99 MB
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Das Handbuch liefert Ihnen Band für Band die Bausteine des modernen Wissens. Es eröffnet Ihnen den Zugang zur molekularen Medizin und zu ihren faszinierenden diagnostischen und therapeutischen Möglichkeiten. Jeder Band stellt Ihnen die modernen Erkenntnisse und das praxisorientierte Wissen zur Verfügung, das Ihren medizinischen Alltag zunehmend bestimmen wird. Ihre Vorteile: Informationsvorsprung auf einem zukunftsweisenden Gebiet der Medizin; Diagnostische und therapeutische Kompetenz in aktuellen Praxisfragen; Sichere Orientierung bereits in der Ausbildung Der erste Band führt Sie systematisch in die allgemeinen Grundlagen der molekularen Medizin ein. Dieses Grundlagenwissen vermittelt Ihnen das Verständnis für die Behandlungskonzepte, die im Mittelpunkt der weiteren Bände stehen. Ein Wissensspeicher, auf den Sie nicht verzichten können. Sichern Sie sich Ihren Informationsvorsprung, und profitieren Sie Band für Band von dem günstigen Vorbestellpreis!