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Biomedical Electron Microscopy

Author: Arvid B. Maunsbach
Publisher: Elsevier
ISBN: 9780080528090
Size: 76.72 MB
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This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs

Electron Microscopy

Author: John Kuo
Publisher: Springer Science & Business Media
ISBN: 1597452947
Size: 17.86 MB
Format: PDF, ePub
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Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. Electron Microscopy Methods and Protocols, Second Edition, offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual. New to this edition are sections covering transmission electron microscopy (TEM), and scanning electron microscopy (SEM).

Microscopic Techniques In Biotechnology

Author: Michael Hoppert
Publisher: John Wiley & Sons
ISBN: 3527605231
Size: 36.47 MB
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Focusing on all current applications, this book presents the various methods as well as their suitability and limitations for a specific question. One particular highlight is the presentation of all basic information on the structure of the relevant objects, thus allowing readers to choose the most suitable applications for any specific problem. They will also find in-depth background information on structure-function relationships, plus descriptions of sample preparations with respect to a particular technique and the necessary equipment. The whole is rounded off with an overview of the future application potential for devices and applications of upcoming interest in biotechnology.

Cellular Electron Microscopy

Author: J. Richard McIntosh
Publisher: Elsevier
ISBN: 9780080475035
Size: 73.70 MB
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Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book’s five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. Updates the numerous technological innovations that have improved the capabilities of electron microscopy Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

Electron Microscopy And Analysis Third Edition

Author: Peter J. Goodhew
Publisher: CRC Press
ISBN: 1482289342
Size: 54.14 MB
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Electron Microscopy and Analysis deals with several sophisticated techniques for magnifying images of very small objects by large amounts - especially in a physical science context. It has been ten years since the last edition of Electron Microscopy and Analysis was published and there have been rapid changes in this field since then. The authors have vastly updated their very successful second edition, which is already established as an essential laboratory manual worldwide, and they have incorporated questions and answers in each chapter for ease of learning. Equally as relevant for material scientists and bioscientists, this third edition is an essential textbook.

Electron Microscopy And Analysis Third Edition

Author: Peter J. Goodhew
Publisher: CRC Press
ISBN: 9780748409686
Size: 53.30 MB
Format: PDF, Docs
View: 5913
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Electron Microscopy and Analysis deals with several sophisticated techniques for magnifying images of very small objects by large amounts - especially in a physical science context. It has been ten years since the last edition of Electron Microscopy and Analysis was published and there have been rapid changes in this field since then. The authors have vastly updated their very successful second edition, which is already established as an essential laboratory manual worldwide, and they have incorporated questions and answers in each chapter for ease of learning. Equally as relevant for material scientists and bioscientists, this third edition is an essential textbook.

Fluorescence Applications In Biotechnology And Life Sciences

Author: Ewa M. Goldys
Publisher: John Wiley & Sons
ISBN: 0470083700
Size: 71.41 MB
Format: PDF, Mobi
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Fluorescence Applications in Biotechnology and the Life Sciences Edited by Ewa M. Goldys A self-contained treatment of the latest fluorescence applications in biotechnology and the life sciences Fluorescence Applications in Biotechnology and the Life Sciences is the first reference in this important subject area to focus specifically on the present applications of fluorescence in molecular and cellular dynamics, biological/medical imaging, proteomics, genomics, and flow cytometry. It is designed to raise awareness of the latest scientific approaches and technologies that may help resolve problems relevant for the industry and the community in areas such as public health, food safety, and environ-mental monitoring. Following an introductory chapter on the basics of fluorescence, the book covers: labeling of cells with fluorescent dyes; genetically encoded fluorescent proteins; nanoparticle fluorescence probes; quantitative analysis of fluorescent images; spectral imaging and unmixing; correlation of light with electron microscopy; fluorescence resonance energy transfer and applications; monitoring molecular dynamics in live cells using fluorescence photo-bleaching; time-resolved fluorescence in microscopy; fluorescence correlation spectroscopy; flow cytometry; fluorescence in diagnostic imaging; fluorescence in clinical diagnoses; immunochemical detection of analytes by using fluorescence; membrane organization; and probing the kinetics of ion pumps via voltage-sensitive fluorescent dyes. With its multidisciplinary approach and excellent balance of research and diagnostic topics, this book will appeal to postgraduate students and a broad range of scientists and researchers in biology, physics, chemistry, biotechnology, bioengineering, and medicine.

The Science Of Laboratory Diagnosis

Author: John Crocker
Publisher: John Wiley & Sons
ISBN: 047085913X
Size: 68.76 MB
Format: PDF, Kindle
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This fully revised and updated edition of The Science of Laboratory Diagnosis provides a concise description of all common laboratory tests available in medical practice with notes on their application, the accuracy of each test, the historical background to the adoption of various tests and their effectiveness in diagnosis. Well illustrated, with clear headings, tables, flow charts and pathology slides, most in full colour Provides an accessible reference book in which relevant information can be found easily Page design facilitates rapid assimilation of principles and key facts All the chapters have been updated and new material has been introduced to cover recently developed techniques, such as fluid-based cytology, telepathology and proteomics The Science of Laboratory Diagnosis, Second Edition is an essential primary reference source for everyone working in a clinical laboratory. This book is essential reading for pathologists, biomedical scientists, medical laboratory scientific officers and all clinicians involved in laboratory research. Reviews of the First Edition: "The text is concise, wide-ranging and easy to digest. The ease of extraction of the important facts make it an ideal source of information for use in a variety of situations from the postgraduate examination to the clinical directors' board meeting." BULLETIN OF THE ROYAL COLLEGE OF PATHOLOGISTS "The editors have done a marvellous job, more than fulfilling their stated aim of producing a volume describing the multidisciplinary state of modern pathology which will be of interest to a wide range of readers. ... I was particularly impressed by the many tables and flow charts, which can be used as aids to decision making." JOURNAL OF CLINICAL PATHOLOGY "This is an excellent book to dip into and get a feel for techniques used in the other disciplines of pathology." ANNALS OF CLINICAL BIOCHEMISTRY

Cellular Imaging

Author: Eric Hanssen
Publisher: Springer
ISBN: 3319689975
Size: 76.69 MB
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This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

Electron Microscopy Of Model Systems

Author:
Publisher: Academic Press
ISBN: 9780123810083
Size: 13.27 MB
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The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. Covers the preparation and analysis of model systems for biological electron microscopy Includes the most popular systems but also organisms that are less frequently used in cell biology Presents the currently most important methods for the preparation of biological specimens First compendium covering the various aspects of sample preparation of very diverse biological systems