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Author: Jeffery M. Becker
Publisher: Academic Press
ISBN: 0323159133
Size: 16.68 MB
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Biotechnology: A Laboratory Course is a series of laboratory exercises demonstrating the in-depth experience and understanding of selected methods, techniques, and instrumentation used in biotechnology. This manual is an outgrowth of an introductory laboratory course for senior undergraduate and first year graduate students in the biological sciences at The University of Tennessee. This book is composed of 19 chapters and begins with some introductory notes on record keeping and safety rules. The first exercises include pH measurement, the use of micropipettors and spectrophotometers, the concept of aseptic technique, and preparation of culture media. The subsequent exercises involve the application of the growth curve, the isolation, purification, and concentration of plasmid DNA from Escherichia coli, and the process of agarose gel electrophoresis. Other exercises include the preparation, purification, and hybridization of probe, the transformation of Saccharomyces cerevisiae, the transformation of E. coli by plasmid DNA, and the principles and applications of protein assays. The final exercises explore the ?-galactosidase assay and the purification and determination of ?-galactosidase in permeabilized yeast cells. This book is of great value to undergraduate biotechnology and molecular biology students.


Author: Jeffrey M. Becker
ISBN: 9780120845620
Size: 10.38 MB
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Asseptic technique and establishing pure cultures: the streak plate and culture transfer. Preparation of culture media. The growth curve. Isolation of plasmid DNA from escherichia coli: the mini-prep. Purification, concentration, and quantitation of DNA. Large-scale isolation of plasmid DNA by column chromatography. Amplification of a lacZ Gene fragment by the polymerase chain reaction. Restriction digestion and agarose gel electrophoresis. Southern transfer. Preparation, purification, and hybridization of probe. Transformation of saccharomyces cerevissiae. Isolation of plasmid from yeast and escherichia coli transformation. Protein assays. Qualitative assay for B-galactosidase in yeast colonies. Determination of B-galactosidase in permeabilized yeast cells. Assay of B-galactosidase in cell extracts. B-galactosidase purification. Western blot: probe of protein blot with antibody to B-galactosidase. Alternative protocols and experiments. Buffer solutions. Preparation of buffers and solutions. Properties of some common concentrated acids and bases. Use of micropipettors. List of cultures. Storage of cultures and DNA. Sterilization methods. Preparation of stock solutions for culture media. Growth in liquid medium. Determination of viable cells. Determination of cell mass. Determination of cell number. Nomenclature of strains. Glassware and plasticware. Preparation of tris and EDTA. Basic rules for handling enzymes. Effects of common contaminants on protein assays. Manufacturers' and distributors' addresses ...

Laboratory Manual For Biotechnology And Laboratory Science

Author: Lisa A. Seidman
Publisher: Benjamin Cummings
ISBN: 9780321644022
Size: 16.29 MB
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Laboratory Manual for Biotechnology provides the basic laboratory skills and knowledge to pursue a career in biotechnology. The manual, written by four biotechnology instructors with over 20 years of teaching experience, incorporates instruction, exercises, and laboratory activities that the authors have been using and perfecting for years. These exercises and activities serve to engage and help you understand the fundamentals of working in a biotechnology laboratory. Building skills through an organized and systematic presentation of materials, procedures, and tasks, the manual will help you explore overarching themes that relate to all biotechnology workplaces. The fundamentals in this manual are critical to the success of research scientists, scientists who develop ideas into practical products, laboratory analysts who analyze samples in forensic, clinical, quality control, environmental, and other testing laboratories.

Calculations For Molecular Biology And Biotechnology

Author: Frank H. Stephenson
Publisher: Academic Press
ISBN: 0128025980
Size: 71.19 MB
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Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts

Basic Laboratory Calculations For Biotechnology

Author: Lisa A. Seidman
Publisher: Benjamin-Cummings Publishing Company
Size: 41.45 MB
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To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Discusses very common laboratory problems, all applied to real situations. Explores multiple strategies for solving problems for a better understanding of the underlying math. Includes hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of “story problems” relating to real situations in the lab. MARKET: A useful review for biotechnology laboratory professionals.

Dna Science

Author: David A. Micklos
Publisher: CSHL Press
ISBN: 9780879696368
Size: 46.11 MB
Format: PDF
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. . .[A]n excellent molecular biology guide and lab manual for beginners. It is concocted uniquely as one part text that introduces the reader to the scientific concepts and one part well designed and tested laboratories in a friendly format.--"Science Books & Films."

Molecular Biology Techniques

Author: Heather Miller
Publisher: Academic Press
ISBN: 0123855454
Size: 19.31 MB
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Fundamental Laboratory Approaches For Biochemistry And Biotechnology

Author: Alexander J. Ninfa
Publisher: Wiley
ISBN: 9780470087664
Size: 32.40 MB
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Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.