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Centrifugation In Density Gradients

Author: C. A. Price
Publisher: Academic Press
ISBN: 1483282066
Size: 68.98 MB
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Centrifugation in Density Gradients provides information pertinent to the fundamental aspects of density gradient centrifugation. This book discusses the benefits of density gradient centrifugation to membrane-bound particles. Organized into nine chapters, this book begins with an overview of the method of differential or fractional centrifugation. This text then explores the physical basis of density gradient centrifugation. Other chapters deal with the nuts and bolts of density gradient centrifugation, the construction and composition of gradients, the properties and operation of centrifuge systems, and certain arcane but highly useful procedures. This book discusses as well density gradient centrifugation in the analytical ultracentrifuge. The final chapter deals with a collection of protocols for separating particles ranging in size from whole cells to macromolecules. This book is intended to be suitable for readers who need to separate biological particles. Biologists, chemists, biochemists, cytologists, physiologists, scientists, and research workers will also find this book useful.

Centrifugation In Biology And Medical Science

Author: Phillip Sheeler
Publisher: Krieger Publishing Company
Size: 63.68 MB
Format: PDF
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Presents a comprehensive account of the various roles that centrifugation takes in biomedical science and the diversity of instrumentation that is available to the researcher. Specific applications are discussed, noting advantages, limitations and other characteristics that are peculiar to each methodological approach. Deals with the historical evolution of the various centrifugal approaches so that recent developments can be appreciated in perspective.

Calcium Signaling

Author: James W. Putney, Jr.
Publisher: CRC Press
ISBN: 9780849333866
Size: 24.26 MB
Format: PDF
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Arguably no other field of biological research embraces such a diverse array of experimental approaches as does the field of calcium signaling. Not only does it span virtually all conceptual and technical areas of molecular and cell biology, but a number of unique techniques, such as the use of permeabilized cells to study intracellular calcium metabolism, have evolved directly from calcium research. Calcium Signaling explores this fascinating area of research, covering the biophysical, molecular biological, and cell biological techniques involved in the study of calcium signaling. This work examines a broad range of topics-from techniques for imaging and measuring calcium in subcellular compartments, to subcellular fractionation and intracellular calcium stores, and patch clamp investigation of calcium channels. Filled with detailed illustrations, Calcium Signaling addresses those key methodological approaches that are useful to scientists and researchers investigating calcium function and regulation in many diverse areas of biological and medical research.

Biochemistry Biochemistry Solutions Manual

Author: J. Stenesh
Publisher: Springer Science & Business Media
ISBN: 9780306457333
Size: 75.19 MB
Format: PDF
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The ideal foundation of a one-semester course for undergraduate students, Stenesh's Biochemistry presents the basic body of biochemical knowledge and a thorough exposition of fundamental biochemical concepts. Carefully balancing primary and secondary topics, this introductory text covers the essentials in proper depth to establish a firm foundation for further study. Superior to any other first level text available, Stenesh's Biochemistry features: clear writing, thorough explanations, and precise definitions. comprehensive study sections for all chapters, consisting of both review-type questions and calculation-type problems, graded by difficulty and including answers selected reading lists concise chapter summaries two-color text 529 illustrations a separate chapter on bioenergetics, and an extensive index. Four appendixes review acid-base calculations, the principles of organic chemistry, the tools of biochemistry, and oxidation-reduction reactions, and a separate Solutions Manual presents step-by-step answers to problems.


Author: David Rickwood
Publisher: John Wiley & Son Limited
Size: 74.83 MB
Format: PDF, ePub
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Centrifugation D. Rickwood, T.C. Ford and J. Steensgaard Centrifugation is at the center of most fractionation methods in a wide range of disciplines. This volume provides the core data required by researchers carrying out any centrifugal separation, whether working with cells, organelles or macromolecules. The Essential Data Series provides rapid access to the core data required by researchers on a daily basis, in convenient pocket-sized volumes.