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Centrosome And Centriole

Author:
Publisher: Academic Press
ISBN: 0128026383
Size: 33.75 MB
Format: PDF
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This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Centrosomes And Spindle Pole Bodies

Author: Robert E. Palazzo
Publisher: Gulf Professional Publishing
ISBN: 9780125439855
Size: 18.14 MB
Format: PDF, ePub, Docs
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Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other cell functions are also included, and a wide variety of experimental systems for analyzing these organelles are presented. Detailed protocols for experiments are contained in each chapter for researchers to perform in their own labs. This volume outlines key methodologies used to analyze centrosomes and spindle pole bodies, their replication, and reproduction in the clear, well-illustrated style of the Methods in Cell Biology series.

Mitosis And Meiosis

Author:
Publisher: Academic Press
ISBN: 9780080859590
Size: 76.58 MB
Format: PDF, ePub, Mobi
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Mitosis and Meiosis details the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle. Chapters Contain Information On: * How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies * Techniques to best image fluorescent and nonfluorescent tagged dividing cells * The use and action of mitoclastic drugs * How to generate antibodies to mitotic components and inject them into cells * Methods that can also be used to obtain information on cellular processes in nondividing cells

Microtubules In Vitro

Author: John J. Correia
Publisher: Academic Press
ISBN: 0124078885
Size: 37.82 MB
Format: PDF
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There continues to be intense interest in the microtubule cytoskeleton; the assembly, structure and regulation of microtubules; and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This 2e brings together current research and protocols in the field of microtubules in vitro and will serve as a valuable tool for cell biologists, biophysicists and pharmacologists who study the microtubule cytoskeleton, as well as for researchers in the biomedical and biotechnology communities with interest in developing drugs that target microtubules, MAPS and motors. Chapters reflect experimental procedures and new developments in the field of microtubule in vitro research Combines classical approaches and modern technologies Presents easy-to-use protocols and thorough background information, compiled by leaders in the field

Medical Cell Biology

Author: Steven R Goodman
Publisher: Academic Press
ISBN: 9780080919317
Size: 61.67 MB
Format: PDF, Kindle
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Medical Cell Biology, Third Edition, focuses on the scientific aspects of cell biology important to medical students, dental students, veterinary students, and prehealth undergraduates. With its National Board-type questions, this book is specifically designed to prepare students for this exam. The book maintains a concise focus on eukaryotic cell biology as it relates to human and animal disease, all within a manageable 300-page format. This is accomplished by explaining general cell biology principles in the context of organ systems and disease. This updated version contains 60% new material and all new clinical cases. New topics include apoptosis and cell death from a neural perspective; signal transduction as it relates to normal and abnormal heart function; and cell cycle and cell division related to cancer biology. 60% New Material! New Topics include: Apoptosis and cell dealth from a neural perspective Signal transduction as it relates to normal and abnormal heart function Cell cycle and cell division related to cancer biology All new clinical cases Serves as a prep guide to the National Medical Board Exam with sample board-style questions (using Exam Master(R) technology): www.exammaster.com Focuses on eukaryotic cell biology as it related to human disease, thus making the subject more accessible to pre-med and pre-health students

The Centrosome In Cell Replication And Early Development

Author:
Publisher: Elsevier
ISBN: 9780080488622
Size: 72.15 MB
Format: PDF, ePub
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Centrosomes play an integral role in the growth of cells and the ultimate development of many animals, and sometimes plants. In addition to the normal growth of cells, centrosomes can also play a key role in the spread of cancer and are of increased interest to both the genetics and oncology communities. Volume 49 of Current Topics in Developmental Biology will present all known research surrounding the centrosome, across a variety of systems, will be well referenced, and speculate where the research is headed. Discusses centrosomes and cancer, centrosomes and early development, and molecular biology of the centrosome Heavily illustrated, with many color figures Chapters written by international leaders in the field

Case Studies In Cell Biology

Author: Merri Lynn Casem
Publisher: Academic Press
ISBN: 0128016841
Size: 72.41 MB
Format: PDF, Docs
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Case Studies in Cell Biology presents real world scenarios to help readers use science process and reasoning skills. The case studies require application and analyzation of concepts beyond rote memory of biological concepts. The book is based on the student learning outcomes from the American Society for Cell Biology, offering practical application for both the classroom and research laboratory. Guides the reader in applying knowledge directly to real world scenarios Includes case studies to bridge foundational cell biological concepts to translational science Aids students in synthesizing information and applying science processes

Caenorhabditis Elegans

Author: Joel H. Rothman
Publisher: Academic Press
ISBN: 0123946204
Size: 49.44 MB
Format: PDF, ePub, Docs
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The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come. Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come Chapters written by leaders in the field Tried and true methods deliver busy researchers a one-stop compendium of essential protocols

Sorting And Recycling Endosomes

Author:
Publisher: Academic Press
ISBN: 0128028718
Size: 34.58 MB
Format: PDF, ePub
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Sorting and Recycling Endosomes provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane. In recent years, the importance of endosomes as a sorting and recycling compartment has become increasingly appreciated. As such, scientists from various fields of cell biology, membrane traffic, and beyond, see the needs to communicate and learn about the methods used to investigate the dynamics and functions of endosomes. This book brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling. This volume covers the approaches necessary to study the key components that mediate the generation and transport of membrane-bounded carriers from the endosomes, and how membrane trafficking machinery is coordinated with cytoskeletons during these processes. In addition to studies carried out in mammalian cells, other model systems such as worm and yeast are also included. Provides the latest information on endosomes, the receiving compartment for endocytosed cargos, and the donor compartment and sorting station for cargos designated to lysosomes, Golgi, or plasma membrane. Covers an increasingly appreciated field in cell biology Includes both established and new technologies Brings together specialists from the field who contribute their expertise on a broad range of biomedical topics that will provide ideal reading for researchers interested in endosomal sorting and recycling