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Dna Isolation And Sequencing

Author: Bruce A. Roe
Publisher: Wiley-Blackwell
ISBN: 9780471963240
Size: 29.90 MB
Format: PDF, Docs
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DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequencing and purification. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

Molecular Biology Of The Cell

Author: Bruce Alberts
Publisher: Garland Science
ISBN: 1317563743
Size: 65.12 MB
Format: PDF, ePub, Docs
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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.

Nucleic Acids

Author: Botho Bowien
Publisher: Amer Scientific Pub
ISBN: 9781588830180
Size: 22.33 MB
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Nucleic Acids Isolation Methods is part of a book series entitled Genome Science and Nucleic Acids. The volume Nucleic Acids Isolation Methods is devoted to preparation of genomic DNA, plasmid DNA, viral nucleic acids, and RNA. Emphasis is also put on stabilization of nucleic acids prior to isolation, scale-up of plasmid isolation, and DNA cleanup from gels, PCR, sequencing, etc. A number of specific medical and forensic applications are detailed such as preparation of pathogen nucleic acids for real-time PCR analysis, extraction of DNA from paraffin embedded bone marrow trephine biopsies, and isolation of nucleic acids from tissue, blood, semen, hair, and saliva for DNA fingerprints, RELP, and PCR analyses. This book contains 10 state-of-the-art review chapters written by 20 renowned experts from leading research institutions, industry and a criminal investigation department. Detailed description of methods as well as their theoretical background makes this volume an essential reference for specialists in academia, research institutions, and industry and also a valuable manual for newcomers to this field.

Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition

Author: Leland J. Cseke
Publisher: CRC Press
ISBN: 9781420041712
Size: 69.42 MB
Format: PDF
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Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Recombinant Dna Methodology

Publisher: Academic Press
ISBN: 1483295974
Size: 51.69 MB
Format: PDF
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Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels. These attributes lead to the solution of complex biological problems and the production of new and better products in the areas of medicine, agriculture, and industry. Recombinant DNA Methodology, a volume in the Selected Methods in Enzymology series produced in benchtop format, contains a selection of key articles from Volumes 68, 100, 101, 153, 154, and 155 of Methods in Enzymology. The essential and widely used procedures provided at an affordable price will be an invaluable aid to the graduate student and the researcher. Enzymes in DNA research DNA isolation, hybridization, and cloning DNA sequence analysis cDNA cloning Gene products Identification of cloned genes and mapping of genes Monitoring cloned gene expression Cloning and transferring of genes into yeast cells Cloning and transferring of genes into plant cells Cloning and transferring of genes into animal cells Site-directed mutagenesis Protein engineering Expression vectors

Basic Techniques In Molecular Biology

Author: Stefan Surzycki
Publisher: Springer Science & Business Media
ISBN: 3642569684
Size: 54.73 MB
Format: PDF, Mobi
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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Basic Methods In Molecular Biology

Author: Leonard Davis
Publisher: Elsevier
ISBN: 044460149X
Size: 65.14 MB
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Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.

Essentials Of Medical Biochemistry

Author: Chung-Eun Ha
Publisher: Academic Press
ISBN: 9780080916880
Size: 13.58 MB
Format: PDF, Kindle
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Expert biochemist N.V. Bhagavan’s new work condenses his successful Medical Biochemistry texts along with numerous case studies, to act as an extensive review and reference guide for both students and experts alike. The research-driven content includes four-color illustrations throughout to develop an understanding of the events and processes that are occurring at both the molecular and macrolecular levels of physiologic regulation, clinical effects, and interactions. Using thorough introductions, end of chapter reviews, fact-filled tables, and related multiple-choice questions, Bhagavan provides the reader with the most condensed yet detailed biochemistry overview available. More than a quick survey, this comprehensive text includes USMLE sample exams from Bhagavan himself, a previous coauthor. * Clinical focus emphasizing relevant physiologic and pathophysiologic biochemical concepts * Interactive multiple-choice questions to prep for USMLE exams * Clinical case studies for understanding basic science, diagnosis, and treatment of human diseases * Instructional overview figures, flowcharts, and tables to enhance understanding

Modern Surgical Care

Author: Thomas A. Miller
Publisher: CRC Press
ISBN: 142001658X
Size: 53.91 MB
Format: PDF
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Completely updated with contributions by world leaders in surgery and the surgery specialties, this reference assists surgeons in the diagnosis and treatment of patients by considering disease as a derangement of normal physiology, thus allowing the surgeon to correlate the appropriate use of laboratory and radiologic modalities. Arranged according to specific organ systems, the book is easily accessible and reflects the impact that scientific discoveries and technical advances have had on our understanding of the physiologic processes in surgical patients.