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Dna Isolation And Sequencing

Author: Bruce A. Roe
Publisher: Wiley-Blackwell
ISBN: 9780471963240
Size: 32.77 MB
Format: PDF, Kindle
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DNA isolation and sequencing are core activities in molecular biology. This concise protocol book provides comprehensive practical guidance on the major methods available, from extraction and restriction digestion, to small and large scale isolation, sequencing and purification. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books: introduce the most commonly used methods, explain the advantages and disadvantages of the methods, and give advice on which procedure to use, provide easy to follow step-by-step protocols, with experimental notes and tips on where to pause, plus information on safety and suppliers.

Human Molecular Biology Laboratory Manual

Author: Stefan Surzycki
Publisher: John Wiley & Sons
ISBN: 0470999241
Size: 64.54 MB
Format: PDF, Docs
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Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual’s distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use

Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition

Author: Leland J. Cseke
Publisher: CRC Press
ISBN: 9781420041712
Size: 35.82 MB
Format: PDF, ePub
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Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Modern Surgical Care

Author: Thomas A. Miller
Publisher: CRC Press
ISBN: 142001658X
Size: 79.76 MB
Format: PDF, Docs
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Completely updated with contributions by world leaders in surgery and the surgery specialties, this reference assists surgeons in the diagnosis and treatment of patients by considering disease as a derangement of normal physiology, thus allowing the surgeon to correlate the appropriate use of laboratory and radiologic modalities. Arranged according to specific organ systems, the book is easily accessible and reflects the impact that scientific discoveries and technical advances have had on our understanding of the physiologic processes in surgical patients.

Fundamentals Of Food Biotechnology

Author: Byong H. Lee
Publisher: John Wiley & Sons
ISBN: 1118384911
Size: 25.99 MB
Format: PDF, ePub
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Food biotechnology is the application of modern biotechnologicaltechniques to the manufacture and processing of food, for examplethrough fermentation of food (which is the oldest biotechnologicalprocess) and food additives, as well as plant and animal cellcultures. New developments in fermentation and enzyme technologicalprocesses, molecular thermodynamics, genetic engineering, proteinengineering, metabolic engineering, bioengineering, and processesinvolving monoclonal antibodies, nanobiotechnology and quorumsensing have introduced exciting new dimensions to foodbiotechnology, a burgeoning field that transcends many scientificdisciplines. Fundamentals of Food Biotechnology, 2nd edition is basedon the author’s 25 years of experience teaching on a foodbiotechnology course at McGill University in Canada. The book willappeal to professional food scientists as well as graduate andadvanced undergraduate students by addressing the latest excitingfood biotechnology research in areas such as genetically modifiedfoods (GMOs), bioenergy, bioplastics, functionalfoods/nutraceuticals, nanobiotechnology, quorum sensing andquenching. In addition, cloning techniques for bacterial and yeastenzymes are included in a “New Trends and Tools”section and selected references, questions and answers appear atthe end of each chapter. This new edition has been comprehensively rewritten andrestructured to reflect the new technologies, products and trendsthat have emerged since the original book. Many new aspectshighlight the short and longer term commercial potential of foodbiotechnology.

Molecular Medical Biochemistry

Author: J. P. Luzio
Publisher: Cambridge University Press
ISBN: 9780521278287
Size: 57.15 MB
Format: PDF, Mobi
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The many exciting developments in cell and molecular biology in recent years have important implications for the practice of medicine. The purpose of this new text is to bring these developments to the attention of undergraduates taking courses in biochemistry. The emphasis is on topics (membrane structure and function, hormone action and secretion) where current knowledge of macromolecular and cellular structure and function is likely to contribute most to understanding the underlying pathology of common multifactorial diseases. The book also outlines the basic features of recombinant DNA techniques and the impact they are having in the diagnosis, treatment and knowledge of disease processes.

E Coli Plasmid Vectors

Author: Nicola Casali
Publisher: Springer Science & Business Media
ISBN: 9781592594092
Size: 26.90 MB
Format: PDF, ePub, Docs
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The authors present a comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, 1 vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids and the use of reporter genes, are also described.