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Essential Ion Channel Methods

Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780123822376
Size: 21.33 MB
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The rapid growth of interest and research activity in ion channels is indicative of their fundamental importance in the maintenance of the living state. This volume was prepared with a view toward providing a sampling of the range of molecular and physical methods that are significant for the study of ion channels. As part of the Reliable Lab Solutions series, Essential Ion Channel Methods brings together chapters from volumes 293 and 294 of Methods in Enzymology. The chapters have been selected by the editor and updated, when possible, by their original authors to include new research and references. The result is a set of chapters which make use of graphics, comparisons to other methods, and provide tricks and approaches that make it possible to adapt methods to other systems. Methods are presented in a fashion that allows their replication by individuals new to the field, yet providing valuable information for seasoned investigators. Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with graphics and easy to follow methods Loaded with detailed protocols developed and used by leaders in the field Refines, organizes and updates popular methods from one of our top selling series, Methods in Enzymology

Essential Bioimaging Methods

Author: P. Michael Conn
Publisher: Academic Press
ISBN: 9780080963426
Size: 26.57 MB
Format: PDF, Docs
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Need a tested, reliable method that works? Put Essential Bioimaging Methods to work for you. Editor Michael Conn has hand-picked the most robust methods from his previously-published volumes in the Methods in Enzymology series. Many of these methods have been briefly updated by the authors that created them and use them in their research, and this book further refines and organizes existing content and focuses on methods that work, including MRI, fMRI, PET, Microscopic optical imaging and other. Part of the Reliable Lab Solution series, this volume provides clear advice and explicit protocols, providing updates to classic, tried-and-true methods and an essential addition to the bookshelf or workbench of any researcher in the field. * Highlights usefulness of techniques in basic research detailing MRI imaging of small animals, fMRI of Macaque monkeys, and baboon model of reperfused stroke * Built from volumes in the flagship brand, Methods in Enzymology * Provides tricks, tips and different approaches

Contemporary Enzyme Kinetics And Mechanism

Author:
Publisher: Academic Press
ISBN: 9780123847447
Size: 35.80 MB
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Kinetic studies of enzyme action provide powerful insights into the underlying mechanisms of catalysis and regulation. These approaches are equally useful in examining the action of newly discovered enzymes and therapeutic agents. Contemporary Enzyme Kinetics and Mechanism, Second Edition presents key articles from Volumes 63, 64, 87, 249, 308 and 354 of Methods in Enzymology. The chapters describe the most essential and widely applied strategies. A set of exercises and problems is included to facilitate mastery of these topics. The book will aid the reader to design, execute, and analyze kinetic experiments on enzymes. Its emphasis on enzyme inhibition will also make it attractive to pharmacologists and pharmaceutical chemists interested in rational drug design. Of the seventeen chapters presented in this new edition, ten did not previously appear in the first edition. Transient kinetic approaches to enzyme mechanisms Designing initial rate enzyme assay Deriving initial velocity and isotope exchange rate equations Plotting and statistical methods for analyzing rate data Cooperativity in enzyme function Reversible enzyme inhibitors as mechanistic probes Transition-state and multisubstrate inhibitors Affinity labeling to probe enzyme structure and function Mechanism-based enzyme inactivators Isotope exchange methods for elucidating enzymatic catalysis Kinetic isotope effects in enzyme catalysis Site-directed mutagenesis in studies of enzyme catalysis

Laboratory And Field Testing Of Unsaturated Soils

Author: Alessandro Tarantino
Publisher: Springer Science & Business Media
ISBN: 1402088191
Size: 50.83 MB
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This volume details recent global advances in laboratory and field testing of unsaturated soils. Coverage includes mechanical, hydraulic, and geo-environmental testing and applications of unsaturated soil monitoring to engineering behavior of geo-structures.

Laboratory Quality Management System

Author: World Health Organization
Publisher:
ISBN: 9789241548274
Size: 44.29 MB
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Achieving, maintaining and improving accuracy, timeliness and reliability are major challenges for health laboratories. Countries worldwide committed themselves to build national capacities for the detection of, and response to, public health events of international concern when they decided to engage in the International Health Regulations implementation process. Only sound management of quality in health laboratories will enable countries to produce test results that the international community will trust in cases of international emergency. This handbook was developed through collaboration between the WHO Lyon Office for National Epidemic Preparedness and Response, the United States of America Centers for Disease Control and Prevention (CDC) Division of Laboratory Systems, and the Clinical and Laboratory Standards Institute (CLSI). It is based on training sessions and modules provided by the CDC and WHO in more than 25 countries, and on guidelines for implementation of ISO 15189 in diagnostic laboratories, developed by CLSI. This handbook is intended to provide a comprehensive reference on Laboratory Quality Management System for all stakeholders in health laboratory processes, from management, to administration, to bench-work laboratorians. This handbook covers topics that are essential for quality management of a public health or clinical laboratory. They are based on both ISO 15189 and CLSI GP26-A3 documents. Each topic is discussed in a separate chapter. The chapters follow the framework developed by CLSI and are organized as the "12 Quality System Essentials".

Calcium Techniques

Author: Jan B. Parys
Publisher:
ISBN: 9781621820789
Size: 54.70 MB
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"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--

Ion Selective Electrode Method

Author: A.K. Covington
Publisher: CRC Press
ISBN: 1351082329
Size: 31.36 MB
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The intention was to produce a book which perforce would never be far from the laboratory, although CRC?s use of Handbook in another connection precludes our use of that word in the title.

Handbook Of Ion Channels

Author: Jie Zheng
Publisher: CRC Press
ISBN: 1466551429
Size: 11.49 MB
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The New Benchmark for Understanding the Latest Developments of Ion Channels Ion channels control the electrical properties of neurons and cardiac cells, mediate the detection and response to sensory stimuli, and regulate the response to physical stimuli. They can often interact with the cellular environment due to their location at the surface of cells. In nonexcitable tissues, they also help regulate basic salt balance critical for homeostasis. All of these features make ion channels important targets for pharmaceuticals. Handbook of Ion Channels illustrates the fundamental importance of these membrane proteins to human health and disease. Renowned researchers from around the world introduce the technical aspects of ion channel research, provide a modern guide to the properties of major ion channels, and present powerful methods for modeling ion channel diseases and performing clinical trials for ion channel drugs. Conveniently divided into five parts, the handbook first describes the basic concepts of permeation and gating mechanisms, balancing classic theories and the latest developments. The second part covers the principles and practical issues of both traditional and new ion channel techniques and their applications to channel research. The third part organizes the material to follow the superfamilies of ion channels. This part focuses on the classification, properties, gating mechanisms, function, and pharmacology of established and novel channel types. The fourth part addresses ion channel regulation as well as trafficking and distribution. The final part examines several ion channel-related diseases, discussing genetics, mechanisms, and pharmaceutical advances.

Molecular Cell Biology

Author: Harvey Lodish
Publisher: Macmillan
ISBN: 9780716776017
Size: 42.71 MB
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The sixth edition provides an authoritative and comprehensive vision of molecular biology today. It presents developments in cell birth, lineage and death, expanded coverage of signaling systems and of metabolism and movement of lipids.

Long Walk To Freedom

Author: Nelson Mandela
Publisher: Little, Brown
ISBN: 9780759521049
Size: 61.80 MB
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The book that inspired the major new motion picture Mandela: Long Walk to Freedom. Nelson Mandela is one of the great moral and political leaders of our time: an international hero whose lifelong dedication to the fight against racial oppression in South Africa won him the Nobel Peace Prize and the presidency of his country. Since his triumphant release in 1990 from more than a quarter-century of imprisonment, Mandela has been at the center of the most compelling and inspiring political drama in the world. As president of the African National Congress and head of South Africa's antiapartheid movement, he was instrumental in moving the nation toward multiracial government and majority rule. He is revered everywhere as a vital force in the fight for human rights and racial equality. LONG WALK TO FREEDOM is his moving and exhilarating autobiography, destined to take its place among the finest memoirs of history's greatest figures. Here for the first time, Nelson Rolihlahla Mandela tells the extraordinary story of his life--an epic of struggle, setback, renewed hope, and ultimate triumph.