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Forensic Applications Of High Performance Liquid Chromatography

Author: Shirley Bayne
Publisher: CRC Press
ISBN: 1439882592
Size: 12.39 MB
Format: PDF
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Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective. Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines: The history of HPLC and the theory behind the separation process The requirements for successful analysis and best practice tips The modes of separation and detection most appropriate for forensic science applications HPLC method development and evaluation The quality aspects of laboratory operation Troubleshooting HPLC systems and analyses Applications of HPLC within the field of forensic science Designed as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers. An instructor’s manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption

Forensic Applications Of Gas Chromatography

Author: Michelle Groves Carlin
Publisher: CRC Press
ISBN: 1466507543
Size: 39.82 MB
Format: PDF, ePub
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Several areas of forensic science use the technique of gas chromatography, ranging from fire analysis to the investigation of fraudulent food and perfumes. Covering the essentials of this powerful analytical technique, Forensic Applications of Gas Chromatography explains the theory and shows applications of this knowledge to various realms of forensic science. Topics include: A brief introduction to gas chromatography and its use in forensic science Various components that make up the gas chromatographic instrumentation The theory of the separation process, along with the chemistry underpinning the process Method development, with a specific example of a separation of eight different compounds using a gas chromatography-flame ionization detector Quality assurance and method validation—with information applicable to many types of analytical testing laboratories Troubleshooting in gas chromatography systems New developments in gas chromatography and advances in columns and detectors Real examples supplement the text, along with questions in each chapter. The book includes examples of applications of gas chromatography in drugs, toxicology, fire, paint, food, and fragrance. Each application is presented as an individual case study with specific focus on a particular sample preparation technique. This allows each technique to be discussed with respect to its theory, instrumentation, solvent selection, and function, as appropriate. Each case study provides readers with suitable practical information to allow them to perform experiments in their own laboratory either as part of a practical laboratory class or in a research context. The final chapter provides answers to the questions and encourages further study and discussion.

Advances In Food Authenticity Testing

Author: Gerard Downey
Publisher: Woodhead Publishing
ISBN: 0081002335
Size: 22.65 MB
Format: PDF, Docs
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Advances in Food Authenticity Testing covers a topic that is of great importance to both the food industry whose responsibility it is to provide clear and accurate labeling of their products and maintain food safety and the government agencies and organizations that are tasked with the verification of claims of food authenticity. The adulteration of foods with cheaper alternatives has a long history, but the analytical techniques which can be implemented to test for these are ever advancing. The book covers the wide range of methods and techniques utilized in the testing of food authenticity, including new implementations and processes. The first part of the book examines, in detail, the scientific basis and the process of how these techniques are used, while other sections highlight specific examples of the use of these techniques in the testing of various foods. Written by experts in both academia and industry, the book provides the most up-to-date and comprehensive coverage of this important and rapidly progressing field. Covers a topic that is of great importance to both the food industry and the governmental agencies tasked with verifying the safety and authenticity of food products Presents a wide range of methods and techniques utilized in the testing of food authenticity, including new implementations and processes Highlights specific examples of the use of the emerging techniques and testing strategies for various foods

High Performance Liquid Chromatography

Author: W.J. Lough
Publisher: CRC Press
ISBN: 9780751400762
Size: 44.45 MB
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High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Hplc In Enzymatic Analysis

Author: Edward F. Rossomando
Publisher: John Wiley & Sons
ISBN: 0470111003
Size: 56.84 MB
Format: PDF, Kindle
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The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.

Forensic Chemistry

Author: Max M. Houck
Publisher: Academic Press
ISBN: 0128006242
Size: 29.76 MB
Format: PDF, Docs
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Forensic Chemistry is the first publication to provide coordinated expert content from world-renowned leading authorities in forensic chemistry. Covering the range of forensic chemistry, this volume in the Advanced Forensic Science Series provides up-to-date scientific learning on drugs, fire debris, explosives, instrumental methods, interpretation, and more. Technical information, written with the degreed professional in mind, brings established methods together with newer approaches to build a comprehensive knowledge base for the student and practitioner alike. Like each volume in the Advanced Forensic Science Series, review and discussion questions allow the text to be used in classrooms, training programs, and numerous other applications. Sections on fundamentals of forensic science, history, safety, and professional issues provide context and consistency in support of the forensic enterprise. Forensic Chemistry sets a new standard for reference and learning texts in modern forensic science. Advanced articles written by international forensic chemistry experts Covers the range of forensic chemistry, including methods and interpretation Includes entries on history, safety, and professional issues Useful as a professional reference, advanced textbook, or training review

Tietz Textbook Of Clinical Chemistry And Molecular Diagnostics E Book

Author: Carl A. Burtis
Publisher: Elsevier Health Sciences
ISBN: 1455759422
Size: 80.33 MB
Format: PDF
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As the definitive reference for clinical chemistry, Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th Edition offers the most current and authoritative guidance on selecting, performing, and evaluating results of new and established laboratory tests. Up-to-date encyclopedic coverage details everything you need to know, including: analytical criteria for the medical usefulness of laboratory procedures; new approaches for establishing reference ranges; variables that affect tests and results; the impact of modern analytical tools on lab management and costs; and applications of statistical methods. In addition to updated content throughout, this two-color edition also features a new chapter on hemostasis and the latest advances in molecular diagnostics. Section on Molecular Diagnostics and Genetics contains nine expanded chapters that focus on emerging issues and techniques, written by experts in field, including Y.M. Dennis Lo, Rossa W.K. Chiu, Carl Wittwer, Noriko Kusukawa, Cindy Vnencak-Jones, Thomas Williams, Victor Weedn, Malek Kamoun, Howard Baum, Angela Caliendo, Aaron Bossler, Gwendolyn McMillin, and Kojo S.J. Elenitoba-Johnson. Highly-respected author team includes three editors who are well known in the clinical chemistry world. Reference values in the appendix give you one location for comparing and evaluating test results. NEW! Two-color design throughout highlights important features, illustrations, and content for a quick reference. NEW! Chapter on hemostasis provides you with all the information you need to accurately conduct this type of clinical testing. NEW! Six associate editors, Ann Gronowski, W. Greg Miller, Michael Oellerich, Francois Rousseau, Mitchell Scott, and Karl Voelkerding, lend even more expertise and insight to the reference. NEW! Reorganized chapters ensure that only the most current information is included.

Handbook Of Hplc Second Edition

Author: Danilo Corradini
Publisher: CRC Press
ISBN: 9781420016949
Size: 44.48 MB
Format: PDF, Mobi
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High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the publication of the benchmark first edition twelve years ago. It reports detailed information on fundamental and practical aspects of HPLC related to conventional format and sophisticated novel approaches which have been developed to address a variety of separation problems in different fields. The latest concepts New concepts presented in this edition include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, and capillary electromigration techniques. The book also discusses LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, field-flow fractionation, retention models for ions, and polymer HPLC. Fundamentals and applications The first section of the book explores emerging novel aspects of HPLC and related separation methods based on the differential velocity of analytes in a liquid medium under the action of either an electric field (capillary electromigration techniques) or a gravitational field (field-flow fractionation). The section focusing on applications highlights four significant areas in which HPLC is successfully employed: chiral pharmaceutical, environmental analysis, food analysis, and forensic science. Ideal for a widespread audience Explanatory figures and tables supplement the text and clarify difficult concepts. Extensive references provide gateways to more focused study. Suitable for undergraduates or new practitioners interested in improving their knowledge on the current status and future trends of HPLC, the book is also a critical resource for researchers looking for solutions to complex separation problems or those who currently use HPLC either as an analytical or a preparative scale tool.

Multidimensional Chromatography

Author: Luigi Mondello
Publisher: John Wiley & Sons
ISBN: 9780471988694
Size: 43.76 MB
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Coupled High Performance Liquid Chromatography with High Resolution Gas Chromatography (L. Mondello); Multidimensional High Resolution Gas Chromatography (A.C. Lewis); Orthogonal GC-GC (P.J. Marriott); Coupled-Column Liquid Chromatography (C. Corradini); Supercritical Fluid Techniques Coupled with Chromatographic Techniques (F.M. Lancas); Unified Chromatography: Concepts and Considerations for Multidimensional Chromatography (T.L. Chester); Multidimensional Planar Chromatography (Sz. Nyiredy); Multidimensional Electrodriven Separations (M.M. Degen and V.T. Remcho) APPLICATIONS; Foods, Flavours and Fragrances Applications (G. Dugo et al); Biomedical and Pharmaceutical Applications (G.W. Somsen and G.J. de Jong); Industrial and Polymer Applications (Y.V. Kazakevich and R. LoBrutto); Multidimensional Chromatography in Environmental Analysis (R.M. Marce ); Multidimensional Chromatography in the Oil Industry (J. Beens); Forensic and Toxicological Applications.