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Gene Biotechnology Third Edition

Author: William Wu
Publisher: CRC Press
ISBN: 1439848327
Size: 31.94 MB
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Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book: Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips Addresses the needs of researchers in academic and commercial environments Guides graduate students in designing, implementing, and evaluating experimental projects. Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.

Calculations For Molecular Biology And Biotechnology

Author: Frank H. Stephenson
Publisher: Academic Press
ISBN: 0128025980
Size: 60.49 MB
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Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts

An Introduction To Genetic Engineering

Author: Desmond S. T. Nicholl
Publisher: Cambridge University Press
ISBN: 9781139471787
Size: 14.22 MB
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In this third edition of his popular undergraduate-level textbook, Des Nicholl recognises that a sound grasp of basic principles is vital in any introduction to genetic engineering. Therefore, the book retains its focus on the fundamental principles used in gene manipulation. It is divided into three sections: Part I provides an introduction to the relevant basic molecular biology; Part II, the methods used to manipulate genes; and Part III, applications of the technology. There is a new chapter devoted to the emerging importance of bioinformatics as a distinct discipline. Other additional features include text boxes, which highlight important aspects of topics discussed, and chapter summaries, which include aims and learning outcomes. These, along with key word listings, concept maps and a glossary, will enable students to tailor their study to suit their own learning styles and ultimately gain a firm grasp of a subject that students traditionally find difficult.

Dna And Biotechnology

Author: Molly Fitzgerald-Hayes
Publisher: Academic Press
ISBN: 9780080916354
Size: 50.25 MB
Format: PDF
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Appropriate for a wide range of disciplines, from biology to non-biology, law and nursing majors, DNA and Biotechnology uses a straightforward and comprehensive writing style that gives the educated layperson a survey of DNA by presenting a brief history of genetics, a clear outline of techniques that are in use, and highlights of breakthroughs in hot topic scientific discoveries. Engaging and straightforward scientific writing style Comprehensive forensics chapter Parallel Pedagogic material designed to help both readers and teachers. Highlights in the latest scientific discoveries Outstanding full-color illustration that walk reader through complex concepts

Gene Biotechnology

Author: S. N. Jogdand
ISBN: 9788184885606
Size: 30.97 MB
Format: PDF
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1 From Classical Genetics to Modern Genetics 2 Genetic Engineering and r-DNA Technology 3 Genetic Complexity 4 Enzymes used in Gene Manipulation of DNA and RNA 5 Restriction Endonucleases-Molecular Scalpels 6 Isolation and Purification of DNA 7 Plasmids 8 Isolation and Purification of Plasmid DNA 9 Preparation of RNA 10 Autoradiography 11 Electrophoresis of DNA 12 Pulsed Field Gel Electrophoresis (PFGE) 13 Nucleic Acid Staining 14 Nucleic Acid Labelling 15 Molecular Probes 16 Hybridization Techniques 17 DNA Fingerprinting (DNA Profiling) 18 Restriction Fragment Length Polymorphism (RFLP) 19 Slotting Techniques 20 Vectors 21 Gene Cloning 22 Screening of Recombinants 23 Protoplast Technology - Fusion Techniques 24 DNA-Protein Interactions 25 DNA Libraries - (a) Genomic Library (b) cDNA Library 26 Chromosome Walking and Jumping 27 Site-Directed Mutagenesis 28 DNA Synthesis 29 Techniques of (Chromosome) Gene Mapping 30 DNA Sequencing (Gene Sequencing) 31 Ribozymes 32 Polymerase Chain Reaction (PCR) 33 Random Amplification of Polymorphic DNA (RAPD) 34 Subtraction Hybridization 35 Ribotyping 36 Protein Engineering 37 Strain Imr.;rovement of Industrially Important Organisms 38 Gene Transfer Technologies (DNA Transfer Methods) 39 Transgenic Animals 40 Human Gene Therapy 41 Gene Targeting 42 Antisense Therapy 43 RNA Interference 44 Flow Cytogenetics 45 Human Genome Project - Part I 45 Human Genome Project - Part II 47 Genomics 48 Proteomics 49 Bioinformatics - I (Primer) 50 Bioinformatics - II (Tools and Techniques) 51 Ethical, Legal, Social, Environmental and Health Issues Related to Gene Biotechnology 52 Sythetic Biology Glossary References.

Biotechnology For Beginners

Author: Reinhard Renneberg
Publisher: Academic Press
ISBN: 0128012730
Size: 21.62 MB
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Biotechnology for Beginners, Second Edition, presents the latest information and developments from the field of biotechnology—the applied science of using living organisms and their by-products for commercial development—which has grown and evolved to such an extent over the past few years that increasing numbers of professionals work in areas that are directly impacted by the science. For the first time, this book offers an exciting and colorful overview of biotechnology for professionals and students in a wide array of the life sciences, including genetics, immunology, biochemistry, agronomy, and animal science. This book also appeals to the lay reader without a scientific background who is interested in an entertaining and informative introduction to the key aspects of biotechnology. Authors Renneberg and Demain discuss the opportunities and risks of individual technologies and provide historical data in easy-to-reference boxes, highlighting key topics. The book covers all major aspects of the field, from food biotechnology to enzymes, genetic engineering, viruses, antibodies, and vaccines, to environmental biotechnology, transgenic animals, analytical biotechnology, and the human genome. This stimulating book is the most user-friendly source for a comprehensive overview of this complex field. Provides accessible content to the lay reader who does not have an extensive scientific background Includes all facets of biotechnology applications Covers articles from the most respected scientists, including Alan Guttmacher, Carl Djerassi, Frances S. Ligler, Jared Diamond, Susan Greenfield, and more Contains a summary, annotated references, links to useful web sites, and appealing review questions at the end of each chapter Presents more than 600 color figures and over 100 illustrations Written in an enthusiastic and engaging style unlike other existing theoretical and dry-style biotechnology books

Recombinant Dna Genes And Genomes

Author: James D. Watson
Publisher: Macmillan
ISBN: 9780716728665
Size: 19.94 MB
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This text presents an introduction to the concepts and techniques of recombinant DNA research and their dramatic results. It explores core concepts in molecular biology in a contemporary inquiry-based context, building its coverage around the landmark experiments that redefined our understanding of DNA.

Gene Biotechnology Second Edition

Author: William Wu
Publisher: CRC Press
ISBN: 9780849312885
Size: 80.27 MB
Format: PDF, Kindle
View: 7158
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Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development. Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects. Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.

The Gene Hunters

Author: Calestous Juma
Publisher: Princeton University Press
ISBN: 1400860253
Size: 76.31 MB
Format: PDF, Docs
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The world is on the verge of receiving new life forms that will profoundly and irrevocably change the global economy: the "gene hunters" who first cloned the gene in 1973 are now not only modifying existing species but also creating new plants and animals. Ready or not for such awesome power, the human race has put itself in a position to govern evolution. What will we do with the abilities we now command? asks this broad and stimulating book on the role of plant material in economic development. Writing in a style that is easily understandable even to those with no background in biotechnology, Calestous Juma begins by showing how the importation of plants strengthened the British Empire and brought the United States to global agricultural superiority. He goes on to explore the current international competition for genetic material and the potential impact of biotechnology on the relationship of the developed and developing world. Juma points out that biotechnology poses real dangers to the third world. Often one of the few exportable resources that a developing country possesses is an unusual or rare crop, but biotechnological techniques make possible the cultivation of many such crops outside their natural habitats, potentially eliminating the need to import the crops from the countries in which they grow indigenously. After discussing the threat of biotechnology, Juma comes full circle and points out that it does not have to be a threat. Actually, tremendous benefits could accrue to the third world from biotechnology--if and only if that new technology is adapted to its needs. Originally published in 1989. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Recombinant Dna And Biotechnology

Author: Helen Kreuzer
Publisher: Wiley-Blackwell
ISBN: 9781555811761
Size: 26.45 MB
Format: PDF, ePub, Mobi
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Written in clear, easy–to–understand language, this best–selling reference text and activities manual offers easy–to–implement lessons and classroom activities. Part I covers basic molecular biology, and Part II offers imaginative dry labs and wet labs that can be done by both college and precollege students. Part III is an innovative section addressing the social issues and public concerns of biotechnology. Extensive appendixes provide important background information on basic laboratory techniques and teaching resources, including overhead masters and templates. Adopted by numerous school systems, this unique book is an outgrowth of molecular biology and biotechnology teaching workshops. All of the exercises and lab activities have been extensively tested in the classroom by hundreds of high school teachers. Recombinant DNA and Biotechnology is designed to interest an international teaching audience and will enable all instructors to teach a reasonable amount of molecular biology and genetic engineering to students. No other book makes it so easy or compelling for teachers to incorporate the "new biology" into their biology, biological sciences, or general science curriculum. Recombinant DNA and Biotechnology: A Guide for Teachers will enable college and precollege teachers to plan and conduct an exciting and contemporary course on the basic principles, essential laboratory activities, and relevant social issues and concerns attendant to today′s molecular biology revolution. In addition to the complete text of the student edition, A Guide for Teachers also contains the answers to all discussion questions and extra background information and material on the scientific principles involved.