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Human Stem Cell Manual

Author: Jeanne F. Loring
Publisher: Academic Press
ISBN: 0123854733
Size: 51.86 MB
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This reader-friendly manual provides a practical "hands on" guide to the culture of human embryonic and somatic stem cells. By presenting methods for embryonic and adult lines side-by-side, the authors lay out an elegant and unique path to understanding the science of stem cell practice. The authors begin with a broad-based introduction to the field, and also review legal and regulatory issues and patents. Each experimental strategy is presented with an historical introduction, detailed method, discussion of alternative methods, and common pitfalls. This lab guide for researchers also serves as a textbook for undergraduate and graduate students in laboratory courses. • Offers a comprehensive introduction to stem cell biology and culture for medical and biology researchers investigating diagnostics and treatments for various diseases • Presents a historical introduction, discussion of alternative methods, and common pitfalls for basic and advanced experimental strategies • Includes new chapters devoted to iPS cells and other alternative sources for generating human stem cells written by the scientists who made these breakthroughs

Human Stem Cell Technology And Biology

Author: Gary S. Stein
Publisher: John Wiley & Sons
ISBN: 9780470889893
Size: 65.41 MB
Format: PDF, Docs
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Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Crispr Cas

Author: University Jennifer Doudna
Publisher:
ISBN: 9781621821304
Size: 24.92 MB
Format: PDF
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The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.

Culture Of Human Stem Cells

Author: R. Ian Freshney
Publisher: John Wiley & Sons
ISBN: 9780470167519
Size: 77.60 MB
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This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.

Culture Of Animal Cells

Author: R. Ian Freshney
Publisher: John Wiley & Sons
ISBN: 1118873378
Size: 18.24 MB
Format: PDF, Kindle
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Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made – particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

Manipulating The Mouse Embryo

Author: Brigid Hogan
Publisher:
ISBN: 9780879693848
Size: 43.69 MB
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A comprehensive source of technical guidance for experienced investigators and essential resource for newcomers to mammalian genetics and embryology. This edition (1st ed., 1986) is revised and expanded to incorporate improvements to existing methods as well as new technologies. It contains new sect

A Manual For Primary Human Cell Culture

Author: Jan-Thorsten Schantz
Publisher: World Scientific
ISBN: 9789812387967
Size: 27.45 MB
Format: PDF, Mobi
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As part of the boom in biotechnology, particularly in tissue engineering, primary human cell culture has become a major pillar in academic research and the biopharmaceutical industry. Obtaining a viable culture from a tissue sample and maintaining it for experimental, diagnostic or therapeutic purposes can be quite a challenge. Based on laboratory protocols and practical experience from many years of primary cell culture, this manual presents the basic steps necessary for culturing primary human cells. Written by students for students, the manual serves well as a practical guide to primary human cell culture. The authors have left much space for notes and the design of the manual is such that it can be continuously upgraded and extended. The content of this manual is by no means exhaustive. Protocols for specific cell types, out of over 200 different cell types in the human body, were selected from major tissue groupings in the body. They should serve as a foundation for individual researchersto experiment, explore, and establish niche protocols for their specific needs. Inspired by the practical clinical checklists available to residents and trainees in medicine, the authors have chosen a compact physical format that can fit into the pocket of a lab coat.

Manual Of Pediatric Hematology And Oncology

Author: Philip Lanzkowsky
Publisher: Academic Press
ISBN: 9780123751553
Size: 22.36 MB
Format: PDF, ePub
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Given that there have been considerable advances in the treatment and management of oncologic diseases in children, the fifth edition of this successful clinical manual will be entirely updated to incorporate all current protocols and developments.This edition will be a comprehensive book on patient management, replete with algorithms and flow diagrams on diagnosis and management. Its concise and easy-to-read format will enable readers to make accurate diagnoses and permit them to treat patients without having extensive previous hematologic/oncologic experience. A list of normal values at various ages in children, providing an extremely useful reference for patient management is included. Selected for inclusion in Doody's Core Titles 2013, an essential collection development tool for health sciences libraries Offers a concise, systematic approach to all pediatric hematologic and oncologic disorders in one reference manual Easy-to-read format: multiple tables, charts, and flow-diagrams for diagnosis and management of pediatric hematologic and oncologic disorders Clear presentation by practicing clinicians, who are also academic researchers, of recent developments in molecular genetics, cytogenetics, immunology, transplantation, and biochemistry

Preservation Of Cells

Author: Allison Hubel
Publisher: John Wiley & Sons
ISBN: 1118989848
Size: 71.54 MB
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Pre-freeze processing and characterization -- Formulation and introduction of cryopreservation solutions -- Freezing protocols -- Storage and shipping of frozen cells -- Thawing and post thaw processing -- Post-thaw assessment -- Algorithm-driven protocol optimization -- Protocols

Brutes Or Angels

Author: James T. Bradley
Publisher: University of Alabama Press
ISBN: 0817317880
Size: 53.27 MB
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"With stem cell research, Dolly the cloned sheep, in vitro fertilization, age retardation, and pharmaceutical mind enhancement, humankind is now faced with decisions that it has never before had to consider. The thoughtfulness, or lack of it, that we bring to those decisions will largely determine the future character of the living world. Brutes or Angels will facilitate informed choice making about the personal use of biotechnologies and the formulation of public policies governing their development and use. Ten biotechnologies that impact humans are considered: stem cell research, embryo selection, human genomics, gene therapies, human reproductive cloning, age retardation, cognition enhancement, the engineering of nonhuman organisms, nanobiology, and synthetic biology. With deft and assured use of metaphors, analogies, diagrams, and photographs, James T. Bradley introduces important biological principles and the basic procedures used in biotechnology. Various ethical issues--personhood, personal identity, privacy, ethnic discrimination, distributive justice, authenticity and human nature, and the significance of mortality in the human life cycle--are presented in a clear and unbiased manner. Personal reflection and group dialogue are encouraged by questions at the end of each chapter, making this book not only a general guide to better informed and nuanced thinking on these complex and challenging topics but also an appropriate text for bioethics courses in university science departments and for adult education classes." -- Publisher's description.