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Lipid Droplets

Author: H. Robert Yang
Publisher: Academic Press
ISBN: 0124095216
Size: 65.97 MB
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This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in the field Cutting-edge material

Plant Cell Biology

Author: Randy O. Wayne
Publisher: Academic Press
ISBN: 012814372X
Size: 64.43 MB
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Plant Cell Biology, Second Edition: From Astronomy to Zoology connects the fundamentals of plant anatomy, plant physiology, plant growth and development, plant taxonomy, plant biochemistry, plant molecular biology, and plant cell biology. It covers all aspects of plant cell biology without emphasizing any one plant, organelle, molecule, or technique. Although most examples are biased towards plants, basic similarities between all living eukaryotic cells (animal and plant) are recognized and used to best illustrate cell processes. This is a must-have reference for scientists with a background in plant anatomy, plant physiology, plant growth and development, plant taxonomy, and more. Includes chapter on using mutants and genetic approaches to plant cell biology research and a chapter on -omic technologies Explains the physiological underpinnings of biological processes to bring original insights relating to plants Includes examples throughout from physics, chemistry, geology, and biology to bring understanding on plant cell development, growth, chemistry and diseases Provides the essential tools for students to be able to evaluate and assess the mechanisms involved in cell growth, chromosome motion, membrane trafficking and energy exchange

Caenorhabditis Elegans

Author: Joel H. Rothman
Publisher: Academic Press
ISBN: 0123946204
Size: 23.63 MB
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The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come. Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come Chapters written by leaders in the field Tried and true methods deliver busy researchers a one-stop compendium of essential protocols

Coherent Raman Scattering Microscopy

Author: Ji-Xin Cheng
Publisher: CRC Press
ISBN: 1439867666
Size: 25.11 MB
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The First Book on CRS Microscopy Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues. In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine. Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine—from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

Emc 2008

Author: Anke Aretz
Publisher: Springer Science & Business Media
ISBN: 354085228X
Size: 47.64 MB
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Proceedings of the14th European Microscopy Congress, held in Aachen, Germany, 1-5 September 2008. Jointly organised by the European Microscopy Society (EMS), the German Society for Electron Microscopy (DGE) and the local microscopists from RWTH Aachen University and the Research Centre Jülich, the congress brings together scientists from Europe and from all over the world. The scientific programme covers all recent developments in the three major areas of instrumentation and methods, materials science and life science.

Ultrastructure Of Endocrine Cells And Tissues

Author: P. Motta
Publisher: Springer Science & Business Media
ISBN: 1461338611
Size: 74.42 MB
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Innovative microscopic techniques, introduced during the last two decades, have contributed much to creating a new picture of the dynamic architecture of the cell, which can now be more exactly correlated with specific biochemical and physiopathological events. These developments have led to significant advances in our understanding of the physiomorphological and pathological aspects of the secretory mechanism, as well as the pharmacologic methods used to control, experimentally, the function of exocrine and endocrine glands. The integration of new ultrastructural methods such as freeze-fracture/etching, immunocytochemistry, scanning and high-voltage electron microscopy, cytoautoradiography, etc. , has proven to be of great value when applied to the study of endocrine cells and tissues. Because information on this topic has appeared in a variety of scientific and medical journals, this book: (1) reviews the results of an integrative approach presenting a comprehensive ultrastructural account of the main aspects of the field; (2) points out gaps or controversial topics in our knowledge; and (3) outlines pertinent directions for future research. The chapters, prepared by recognized authorities in the field, present traditional information on the topic in a concise manner and, with a valuable selection of original illustrations, show what the integration of new microscopic methods can contribute to the subject in terms of new concepts. This volume will be useful to cell biologists, anatomists, embryologists, histologists, pharmacologists, pathologists, and, of course, endocrinologists. It will also be of interest to students, practitioners of medicine, and to all others dealing with clinical research and diagnosis.

Occult Atherosclerotic Disease

Author: A-M. Salmasi
Publisher: Springer Science & Business Media
ISBN: 9401134049
Size: 42.45 MB
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Occult atherosclerotic diseases impose great challenges in the cardiovascular practice. Although their pathology is not much different from that of the overt group of diseases, the clinical approach to their diagnoses remains a puzzle. This has mainly emerged as a result of the problems faced in trying to pick up the vulnerable subjects from among the general public. More definitive candidates to be evaluated are those belonging to one of the high risk groups. There are no statistical figures available on the incidence of occult atherosclerotic disease in the "non risky" general public and this is definitely an area that needs further investigation. Atherosclerotic disease whether they are in the cerebral, coronary, renal or lower limb arteries are theoretically interrelated because the basic patho logical changes are usually similar. However there still remain some differ ences which are mainly due to variation in the flow pattern in the arteries. Thus it will be important clinically and from the management point of view to investigate for the presence of occult disease in other arteries if an atherosclerotic disease in a certain artery has been discovered. This approach is of a great significance especially from the preventive point of view in order to avoid catastrophic events which may have resulted from pathology in the other region.