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Lipid Droplets

Author: H. Robert Yang
Publisher: Academic Press
ISBN: 0124095216
Size: 69.57 MB
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This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in the field Cutting-edge material

Molecular Characterization Of Autophagic Responses

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Publisher: Academic Press
ISBN: 0128097949
Size: 30.78 MB
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Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional. Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses Written in an accessible style by renowned experts in the field

Lipids

Author:
Publisher: Academic Press
ISBN: 0123864887
Size: 30.87 MB
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Lipids are a broad group of naturally occurring molecules which includes fats, waxes, sterols, fat-soluble vitamins (such as vitamins A, D, E and K), monoglycerides, diglycerides, phospholipids, and others. The main biological functions of lipids include energy storage, as structural components of cell membranes, and as important signaling molecules. This volume of Methods in Cell Biology covers such areas as Membrane structure and dynamics, Imaging, and Lipid Protein Interactions. It will be an essential tool for researchers and students alike. Covers such areas as membrane structure and dynamics, imaging, and lipid protein interactions An essential tool for researchers and students alike International authors Renowned editors

Plant Cell Biology

Author: Randy O. Wayne
Publisher: Academic Press
ISBN: 9780080921273
Size: 48.98 MB
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Plant Cell Biology is a semester long course for undergraduates and graduate students which integrates mathematics and physics, two years of chemistry, genetics, biochemistry and evolution disciplines. Having taught this course for over ten years, the author uses his expertise to relate the background established in plant anatomy, plant physiology, plant growth and development, plant taxonomy, plant biochemistry, and plant molecular biology courses to plant cell biology. This integration attempts to break down the barrier so plant cell biology is seen as an entrée into higher science. Distinguishing this book from papers that are often used for teaching the subject which use a single plant to demonstrate the techniques of molecular biology, this book covers all aspects of plant cell biology without emphasizing any one plant, organelle, molecule, or technique. Although most examples are biased towards plants, basic similarities between all living eukaryotic cells (animal and plant) are recognized and used to best illustrate for students cell processes. Thoroughly explains the physiological underpinnings of biological processes to bring original insight related to plants Includes examples throughout from physics, chemistry, geology, and biology to bring understanding to plant cell development, growth, chemistry and diseases Provides the essential tools for students to be able to evaluate and assess the mechanisms involved in cell growth, chromosome motion, membrane trafficking, and energy exchange Companion Web site provides support for all plant cell biology courses

Coherent Raman Scattering Microscopy

Author: Ji-Xin Cheng
Publisher: CRC Press
ISBN: 1439867666
Size: 52.47 MB
Format: PDF
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The First Book on CRS Microscopy Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues. In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine. Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine—from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

Building A Cell From Its Component Parts

Author:
Publisher: Academic Press
ISBN: 0128026391
Size: 18.76 MB
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The cell interior is another world that we are only beginning to explore. Although there are a number of approaches for examining the inner workings of the cell, the reductionist approach of building up complexity appeals to many with physical science and engineering backgrounds. This volume of Methods in Cell Biology spans a range of spatial scales from single protein molecules to vesicle and cell sized structures capable of complex behaviors. Contributions include; methods for combining different motors and cytoskeletal components in defined ways to produce more complex behaviors; methods to combine cytoskeletal assemblies with fabricated devices such as chambers or pillar arrays; reconstituting membrane fission and fusion; reconstituting important biological processes that normally take place on membrane surfaces; and methods for encapsulating protein machines within vesicles or droplets. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Emc 2008

Author: Anke Aretz
Publisher: Springer Science & Business Media
ISBN: 354085228X
Size: 76.78 MB
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Proceedings of the14th European Microscopy Congress, held in Aachen, Germany, 1-5 September 2008. Jointly organised by the European Microscopy Society (EMS), the German Society for Electron Microscopy (DGE) and the local microscopists from RWTH Aachen University and the Research Centre Jülich, the congress brings together scientists from Europe and from all over the world. The scientific programme covers all recent developments in the three major areas of instrumentation and methods, materials science and life science.