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Molecular Biology Techniques

Author: Walt Ream
Publisher: Elsevier
ISBN: 0125839901
Size: 14.63 MB
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

Molecular Microbiology Laboratory

Author: Walt Ream
Publisher: Academic Press
ISBN: 012397044X
Size: 25.34 MB
Format: PDF
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Molecular Microbiology Laboratory, second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. Exposes students to the new molecular-based techniques Provides faculty with an authoritative, accessible resource for teaching protocols The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum Based on a successful course for which the author won a teaching award New to this Edition: - Presents a real-world study of bacterial populations in the environment in the final experiment - Provides an overview of molecular biology in a new review chapter - Demonstrates how to design an experiment and how to interpret the results - Covers grant proposal writing and how panels review proposals - Presents guidance on public speaking and preparing PowerPoint presentations - Includes tutorials on three widely used software packages

Molecular Biology Techniques

Author: Heather Miller
Publisher: Academic Press
ISBN: 0123855454
Size: 16.92 MB
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Molecular Biology

Author: Burton E. Tropp
Publisher: Jones & Bartlett Learning
ISBN: 9780763709167
Size: 35.76 MB
Format: PDF, Kindle
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The Third Edition of Molecular Biology: Genes to Proteins is a comprehensive guide through the basic molecular processes and genetic phenomena of both prokaryotic and eukaryotic cells. Designed for undergraduate and first year graduate students, this multi-purpose text has been completely updated to include many important advances made in the field of molecular biology. Maintaining the original structure-function approach evident in the first two editions of David Freifelder's classic text, this book provides students with an accessible overview of molecular biology before moving into more in-depth discussions. Broken into six sections, topics are strategically arranged to cover basic information that is necessary in understanding the more advanced topics focused on in later sections. Section 1 and 2, Protein Structure and Function and Nucleic Acids and Nucleoproteins provide the information required to Understand The chemical basis of molecular biology. Section 3, Genetics and Virology provides information required to Understand The biological basis of molecular biology. Section 4, DNA Metabolism explores DNA replication, repair, and recombination in bacteria, eukaryotes and archaeons. Section 5, RNA Synthesis and Processing examines mechanisms that bacteria, eukaryotes, and archaeons use to synthesize RNA, regulate RNA syntheses, and process transcripts to form mature RNA molecules. Section 6, Protein Synthesis describes the structure and function of the bacterial protein synthetic machinery in bacteria, eukaryotes and archaeons. View Preview Chapters in PDF of Molecular Biology: Genes to Proteins, Third Edition Today! Preview Chapter 1 Preview Chapter 2

Using The Biological Literature

Author: Diane Schmidt
Publisher: CRC Press
ISBN: 9780824741716
Size: 64.79 MB
Format: PDF, Docs
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"Provides an in-depth review of current print and electronic tools for research in numerous disciplines of biology, including dictionaries and encyclopedias, method guides, handbooks, on-line directories, and periodicals. Directs readers to an associated Web page that maintains the URLs and annotations of all major Inernet resources discussed in this edition."

Introduction To Experimental Biophysics Set

Author: Jay Nadeau
Publisher: CRC Press
ISBN: 9781498739726
Size: 17.98 MB
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This set presents wet lab methods for courses in biophysics or molecular biology. It provides a thorough description of modern experimental and analytical techniques used in biological and biophysical research. The author gives practical, step-by-step guidance on instrumentation and experimental design and includes recipes for common solutions and media, lists of important reagents, and a glossary of biological terms. The manual offers a flexible course plan that permits completion of the labs in either a full term or intensive summer course. Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later experiments deal with more advanced, emerging techniques, such as the synthesis and characterization of quantum dots and gold nanoparticles, protein crystallization, and spectroscopic techniques.

Introduction To Experimental Biophysics A Laboratory Guide

Author: Jay L. Nadeau
Publisher: CRC Press
ISBN: 1466557664
Size: 13.87 MB
Format: PDF, ePub, Mobi
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Easily Get Started with Biological Experiments Introduction to Experimental Biophysics - A Laboratory Guide presents wet lab methods for courses in biophysics or molecular biology. A companion to the author’s highly praised An Introduction to Experimental Biophysics: Biological Methods for Physical Scientists, this manual offers a flexible course plan that permits completion of the labs in either a full term or intensive summer course. Tested in a pedagogical setting, the experiments follow a logical progression beginning with a DNA construct. The book starts with the basics of molecular cloning: amplifying and purifying plasmid, plasmid mapping, and using restriction enzymes. Later experiments deal with more advanced, emerging techniques, such as the synthesis and characterization of quantum dots and gold nanoparticles, protein crystallization, and spectroscopic techniques. This accessible guide will help both students and instructors in molecular biology, biophysics, and biomedical engineering. Students will understand how to use a variety of techniques in biological experiments while instructors will get practical guidance on preparing the experiments.

Current Protocols Essential Laboratory Techniques

Author: Sean R. Gallagher
Publisher: Current Protocols
ISBN: 9780470089934
Size: 69.69 MB
Format: PDF
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2008 PROSE award winner for Excellence in Biology and the Life Sciences From the leading branded source for methods in the life sciences, this essential resource for the lab provides every researcher with the skills and understanding of fundamental laboratory procedures to ensure greater success at the bench. It takes the novice researcher from the very basics of reagent preparation to the use of routine instrumentation found in most life science research laboratories around the world. Developed by Current Protocols, the most trusted and recognized source of protocols Teaches new investigators how to perform basic laboratory research techniques Describes solution chemistry and preparation Covers basic laboratory safety Describes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes Teaches how to manage information from lab notebooks, images, literature references, as well as manuscript preparation This book is an invaluable resource for researchers in all areas of the life sciences, especially molecular biology, biotechnology, genetics, and immunology. It is essential reading for undergraduate and graduate students alike and is also of interest for investigators new to life science research.