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Practical Tissue Culture Applications

Author: Karl Maramorosch
Publisher: Elsevier
ISBN: 0323150179
Size: 17.92 MB
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Practical Tissue Culture Applications contains the proceedings of a conference held at the International Laboratory for Research on Animal Diseases in Nairobi, Kenya, August 24-29, 1978. This book aims to describe some of the more important practical applications of in vitro techniques in a simple, easily understandable manner. Organized into three sections, with a total of 27 chapters, this book provides critical reviews, describes various techniques, and presents complete step-by-step methodology. It emphasizes applications pertaining to the health and economy in developing nations. In particular, this book discusses the pitfalls in preparing general purpose culture media, balanced salt solutions, and the procedures followed in the development of modern in vitro techniques. It also describes techniques for cultivation of vertebrate cells and organs; plant tissue culture and its numerous applications; and electron microscopy of cultured cell. This book explains as well virus isolation and identification in cell cultures, mass production of cells for vaccines, and use of cultured cells for drug evaluation. The applications of in vitro techniques to parasitology are explored in numerous chapters of this book. Considering the potential benefit of application of in vitro techniques, this reference material will be of interest both in developed and developing countries.

Plant Tissue Culture

Author: Timir Baran Jha
Publisher: Universities Press
ISBN: 9788173714887
Size: 37.84 MB
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Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed.

Applications Of Plant Cell And Tissue Culture

Author: Gregory R. Bock
Publisher: John Wiley & Sons
ISBN: 0470513667
Size: 19.51 MB
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This work deals with basic plant physiology and cytology, and addresses the practical exploitation of plants, both as crops and as sources of useful compounds produced as secondary metabolites. Covers problems of commercial exploitation, socio-legal aspects of genetic engineering of crop plants, and of the difficulties of marketing natural compunds produced by cells under artificial conditions.

Plant Tissue Culture Manual

Author: K. Lindsey
Publisher: Springer Science & Business Media
ISBN: 9780792320272
Size: 60.42 MB
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The third supplement to the manual expands the coverage of existing sections and introduces the new section Mutant Selection. Future supplements will continue to update existing sections and will also introduce new sections on Secondary Metabolites and Tissue Culture Techniques for Fundamental Studies, maintaining the manual as a practical and up-to-date source of lab bench procedures.

Plant Tissue Culture Development And Biotechnology

Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 1439896143
Size: 12.23 MB
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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology. Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them. Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology. Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences.

Invertebrate Cell Culture Applications

Author: Karl Maramorosch
Publisher: Elsevier
ISBN: 0323158838
Size: 67.68 MB
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Invertebrate Cell Culture Applications assesses the status of invertebrate cell culture at a time when this method can be used to solve problems in a number of diverse disciplines. Organized into seven chapters, this book begins by discussing the development and amino acid requirements of insect cell culture. It then describes the Drosophila tissue culture and chromosomal phenomena in cell lines of this organism. This book also explains the culture conditions regulating the infection of cells by an intracellular microorganism, as well as the replication of arboviruses in arthropod in vitro systems. Lastly, the characteristics, growth requirements, and applications of tick cell culture to parasitology are explored. This book will contribute in solving biomedical and agricultural problems. This reference material will be of special interest to parasitologists, virologists, microbiologists, entomologists, geneticists, medical researchers, and graduate students in related fields of biomedical research.

Plant Cell Culture

Author: R. A. Dixon
Publisher: Oxford University Press
ISBN: 9780199634026
Size: 56.27 MB
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Plant cell culture techniques are being increasingly used in basic research and for plant exploitation in industry (genetic engineering, micropropagation). In this book, internationally recognized experts describe the techniques in use in their laboratories for isolating and manipulating cell cultures, and their central importance in plant biotechnology.

Plant Tissue Culture And Moelcular Markers

Author: Ashwani Kumar
Publisher: I. K. International Pvt Ltd
ISBN: 8189866109
Size: 49.28 MB
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Besides, recently molecular biology has assumed great importance with respect to plant biotechnology. The present book amalgamates all three aspects into one, practical applications of various techniques being the need of the hour. It discusses micropropagation studies on several crop plants, molecular basis of understanding various life processes including molecular basis of somatic embryogenesis and other physiological and biochemical processes having significant biotechnological applications. It also includes in vitro studies of some important plants like Aloe vera, Simmondsia chinensis, Anacyclus pyrethrum and Crataeva nurvala, Arachis hypogaea L., Phoenix dactylifera, Dendrocalamus asper, Asparagus adescendens Roxb., natural products of plant origin with their therapeutic potential and biotechnological production, genome analysis of crop plants with future applications in biotechnology etc.

Plant Tissue Culture Theory And Practice

Author: S.S. Bhojwani
Publisher: Elsevier
ISBN: 0444596151
Size: 69.25 MB
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Now available only in paperback, this book has been described as `` far the most comprehensive book on plant tissue culture...few publications in this field can compare with this book in terms of subject matter covered and literature surveyed. Overall, the book is a fine achievement for Drs. Bhojwani and Razdan. It also serves the authors' avowed purpose of integrating the theoretical and practical aspects of plant tissue culture. If you like a text and a laboratory manual on plant tissue culture combined, this is obviously a book to be considered seriously.'' (Plant Science Bulletin). Plant tissue culture has become an invaluable aid in the field of experimental botany and has many practical applications in agriculture and horticulture. In recognition of its importance in basic and applied areas of plant science, many universities have included this subject in undergraduate and postgraduate courses but find that they lack a suitable introductory text. This book has been written primarily to fill that need. Starting with an introductory history, the book covers such practical aspects as laboratory requirements and media preparation. The authors go on to discuss fundamental aspects of cellular totipotency (e.g. production of haploids, diploids and triploids, and raising new genotypes through single cell culture), in vitro approaches to plant breeding, raising high health plants, micropropagation, and techniques of in vitro storage of germplasm. Profusely illustrated with line drawings and original photographs, the book is further enhanced by the inclusion of a complete bibliography.