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Practical Tissue Culture Applications

Author: Karl Maramorosch
Publisher: Elsevier
ISBN: 0323150179
Size: 47.99 MB
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Practical Tissue Culture Applications contains the proceedings of a conference held at the International Laboratory for Research on Animal Diseases in Nairobi, Kenya, August 24-29, 1978. This book aims to describe some of the more important practical applications of in vitro techniques in a simple, easily understandable manner. Organized into three sections, with a total of 27 chapters, this book provides critical reviews, describes various techniques, and presents complete step-by-step methodology. It emphasizes applications pertaining to the health and economy in developing nations. In particular, this book discusses the pitfalls in preparing general purpose culture media, balanced salt solutions, and the procedures followed in the development of modern in vitro techniques. It also describes techniques for cultivation of vertebrate cells and organs; plant tissue culture and its numerous applications; and electron microscopy of cultured cell. This book explains as well virus isolation and identification in cell cultures, mass production of cells for vaccines, and use of cultured cells for drug evaluation. The applications of in vitro techniques to parasitology are explored in numerous chapters of this book. Considering the potential benefit of application of in vitro techniques, this reference material will be of interest both in developed and developing countries.

Plant Tissue Culture

Author: Timir Baran Jha
Publisher: Universities Press
ISBN: 9788173714887
Size: 24.50 MB
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Plant Tissue Culture In One Form Or Another Has Become One Of The Most Promising Branches Of Plant Science. Arising From The Totipotency Of Plant Cells, It Now Occupies A Key Position In Plant Breeding, Plant Propagation And Plant Biotechnology.Plant Tissue Culture - Basic And Applied Brings To The Student Accessible, Up-To-Date Information On This Subject. Basic Knowledge Of Tissue Culture Methods Such As Isolation Of Suitable Tissues From The Mother Plant, Maintenance Of The Tissues Under In Vitro Condition In An Undifferentiated Or De-Differentiated Stage, Methods Of Genetic Engineering And Gene Transfer, Chromosomal Studies And The Handling Of In Vitro Micro Plants Are Described In Detail In This Book. Similarly, Application Aspects Of Micropropagation, Haploid Cell Culture, Protoplast Culture, Embryo Culture, Somatic Embryogenesis And Artificial Seeds Are Also Discussed.

Plant Tissue Culture Development And Biotechnology

Author: Robert N. Trigiano
Publisher: CRC Press
ISBN: 1439896143
Size: 30.80 MB
Format: PDF, Kindle
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Under the vast umbrella of Plant Sciences resides a plethora of highly specialized fields. Botanists, agronomists, horticulturists, geneticists, and physiologists each employ a different approach to the study of plants and each for a different end goal. Yet all will find themselves in the laboratory engaging in what can broadly be termed biotechnology. Addressing a wide variety of related topics, Plant Tissue Culture, Development, and Biotechnology gives the practical and technical knowledge needed to train the next generation of plant scientists regardless of their ultimate specialization. With the detailed perspectives and hands-on training signature to the authors’ previous bestselling books, Plant Development and Biotechnology and Plant Tissue Culture Concepts and Laboratory Exercises, this book discusses relevant concepts supported by demonstrative laboratory experiments. It provides critical thinking questions, concept boxes highlighting important ideas, and procedure boxes giving precise instruction for experiments, including step-by-step procedures, such as the proper microscope use with digital photography, along with anticipated results, and a list of materials needed to perform them. Integrating traditional plant sciences with recent advances in plant tissue culture, development, and biotechnology, chapters address germplasm preservation, plant growth regulators, embryo rescue, micropropagation of roses, haploid cultures, and transformation of meristems. Going beyond the scope of a simple laboratory manual, this book also considers special topics such as copyrights, patents, legalities, trade secrets, and the business of biotechnology. Focusing on plant culture development and its applications in biotechnology across a myriad of plant science specialties, this text uses a broad range of species and practical laboratory exercises to make it useful for anyone engaged in the plant sciences.

Introduction To Plant Cell Tissue And Organ Culture

Author: SUNIL D. PUROHIT
Publisher: PHI Learning Pvt. Ltd.
ISBN: 8120346777
Size: 80.71 MB
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Designed primarily as a text for undergraduate and postgraduate students of Botany and Plant Biotechnology, the book discusses the theoretical aspects and modern applications of plant cell, tissue and organ culture. Written with the aim of providing up-to-date information on the subject, and focused on the concept of commercialization of plant cell culture, the contents have been presented with clarity. The book not only discusses the theoretical aspects of plant tissue culture but also emphasizes the art of its practice. It also provides a systematic explanation of asepsis and methods of sterilization, plant tissue culture techniques, culture of reproductive structures, plant tissue culture in germplasm conservation, its applications in the industry and plant pathology and operation and management of greenhouse hardening unit. In addition, it discusses in vitro propagation of plants (micropropagation) with a series of case studies pertaining to tree species and horticultural crops. Besides students, the book will also prove to be useful for researchers, scholars and teachers.

Plant Tissue Culture An Introductory Text

Author: Sant Saran Bhojwani
Publisher: Springer Science & Business Media
ISBN: 8132210263
Size: 52.78 MB
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Plant tissue culture (PTC) is basic to all plant biotechnologies and is an exciting area of basic and applied sciences with considerable scope for further research. PTC is also the best approach to demonstrate the totipotency of plant cells, and to exploit it for numerous practical applications. It offers technologies for crop improvement (Haploid and Triploid production, In Vitro Fertilization, Hybrid Embryo Rescue, Variant Selection), clonal propagation (Micropropagation), virus elimination (Shoot Tip Culture), germplasm conservation, production of industrial phytochemicals, and regeneration of plants from genetically manipulated cells by recombinant DNA technology (Genetic Engineering) or cell fusion (Somatic Hybridization and Cybridization). Considerable work is being done to understand the physiology and genetics of in vitro embryogenesis and organogenesis using model systems, especially Arabidopsis and carrot, which is likely to enhance the efficiency of in vitro regeneration protocols. All these aspects are covered extensively in the present book. Since the first book on Plant Tissue Culture by Prof. P.R. White in 1943, several volumes describing different aspects of PTC have been published. Most of these are compilation of invited articles by different experts or proceedings of conferences. More recently, a number of books describing the Methods and Protocols for one or more techniques of PTC have been published which should serve as useful laboratory manuals. The impetus for writing this book was to make available a complete and up-to-date text covering all basic and applied aspects of PTC for the students and early-career researchers of plant sciences and plant / agricultural biotechnology. The book comprises of nineteen chapters profusely illustrated with self-explanatory illustrations. Most of the chapters include well-tested protocols and relevant media compositions that should be helpful in conducting laboratory experiments. For those interested in further details, Suggested Further Reading is given at the end of each chapter, and a Subject and Plant Index is provided at the end of the book.

Applications Of Plant Cell And Tissue Culture

Author: CIBA Foundation Symposium
Publisher: John Wiley & Sons
ISBN: 0470513667
Size: 45.32 MB
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This work deals with basic plant physiology and cytology, and addresses the practical exploitation of plants, both as crops and as sources of useful compounds produced as secondary metabolites. Covers problems of commercial exploitation, socio-legal aspects of genetic engineering of crop plants, and of the difficulties of marketing natural compunds produced by cells under artificial conditions.

Plant Cell Culture

Author: R. A. Dixon
Publisher: Oxford University Press
ISBN: 9780199634026
Size: 24.53 MB
Format: PDF
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Plant cell culture techniques are being increasingly used in basic research and for plant exploitation in industry (genetic engineering, micropropagation). In this book, internationally recognized experts describe the techniques in use in their laboratories for isolating and manipulating cell cultures, and their central importance in plant biotechnology.

Plant Tissue Culture Manual

Author: K. Lindsey
Publisher: Springer Science & Business Media
ISBN: 9780792320272
Size: 32.69 MB
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The third supplement to the manual expands the coverage of existing sections and introduces the new section Mutant Selection. Future supplements will continue to update existing sections and will also introduce new sections on Secondary Metabolites and Tissue Culture Techniques for Fundamental Studies, maintaining the manual as a practical and up-to-date source of lab bench procedures.

Introduction To Cell And Tissue Culture

Author: Jennie P. Mather
Publisher: Springer Science & Business Media
ISBN: 0585275718
Size: 15.64 MB
Format: PDF, Docs
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It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Modern Applications Of Plant Biotechnology In Pharmaceutical Sciences

Author: Saurabh Bhatia
Publisher: Academic Press
ISBN: 0128024984
Size: 21.63 MB
Format: PDF
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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions