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Tetrahymena Thermophila

Author: Kathleen Collins
Publisher: Academic Press
ISBN: 0123859670
Size: 63.46 MB
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This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology. Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field. With cutting edge material, this comprehensive collection is intended to guide researchers of Tetrahymena thermophila for years to come. Covers sections on systems perspectives and operating principles Chapters are written by experts in the field Cutting-edge material, making this a truly comprehensive collection

Tetrahymena Thermophila

Author:
Publisher: Academic Press
ISBN: 9780080524955
Size: 77.85 MB
Format: PDF
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Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice. Contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena Indispensible for both the novice and the experienced researcher Overviews the history, cell biology, and genetics of the organism Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope Illustrates how the methods can be applied to solve various cell biological problems Reviews recently developed strategies for altering gene expression

Methods In Cilia And Flagella

Author:
Publisher: Academic Press
ISBN: 0128026405
Size: 17.80 MB
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The goal of this book is to collect methods and protocols for studying cilia in a wide range of different cell types, so that researchers from many fields of biology can start exploring the role of cilia in their own system. Chapters are written by experts in the field Cutting-edge material

Electron Microscopy Of Model Systems

Author:
Publisher: Academic Press
ISBN: 9780123810083
Size: 55.72 MB
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The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. Covers the preparation and analysis of model systems for biological electron microscopy Includes the most popular systems but also organisms that are less frequently used in cell biology Presents the currently most important methods for the preparation of biological specimens First compendium covering the various aspects of sample preparation of very diverse biological systems

Quantitative Imaging In Cell Biology

Author:
Publisher: Academic Press
ISBN: 0124202012
Size: 41.81 MB
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This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Cilia Model Organisms And Intraflagellar Transport

Author:
Publisher: Academic Press
ISBN: 9780123813787
Size: 55.41 MB
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Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures. * Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components * Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms

Cilia Motors And Regulation

Author:
Publisher: Academic Press
ISBN: 9780080961071
Size: 55.95 MB
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Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

The Zebrafish Genetics Genomics And Transcriptomics

Author:
Publisher: Academic Press
ISBN: 0128034882
Size: 56.90 MB
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The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish. Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome. Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies Presents chapters written by experts in the field Contains cutting-edge material on the topic

Cilia And Flagella

Author:
Publisher: Academic Press
ISBN: 9780080859453
Size: 11.85 MB
Format: PDF, Kindle
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Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella. Key Features * A complete guide containing more than 80 concise technical chapters friendly to both the novice and experienced researcher * Covers protocols for cilia and flagella across systems and species from Chlamydomonas and Euglena to mammals * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time, including microscopy, electrophoresis, and PCR * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures

Cell Biological Applications Of Confocal Microscopy

Author:
Publisher: Academic Press
ISBN: 0080925359
Size: 69.14 MB
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This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures