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The Pcr Revolution

Author: Stephen A. Bustin
Publisher: Cambridge University Press
ISBN: 0521882311
Size: 14.84 MB
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Examines the latest innovations and the overall impact of PCR on areas of molecular research.

Pcr Troubleshooting And Optimization

Author: Suzanne Kennedy
Publisher: Horizon Scientific Press
ISBN: 1904455727
Size: 50.94 MB
Format: PDF, Docs
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The polymerase chain reaction (PCR) is a fundamental tool in scientific research and clinical testing. Real-time PCR, combining both amplification and detection in one instrument, is a rapid and accurate method for nucleic acid detection and quantification. Although PCR is a very powerful technique, the results achieved are valid only if the appropriate controls have been employed. In addition, proper optimization of PCR conditions is required for the generation of specific, repeatable, reproducible, and sensitive data. This book discusses the strategies for preparing effective controls and standards for PCR, when they should be employed, and how to interpret the information they provide. It highlights the significance of optimization for efficiency, precision, and sensitivity of PCR methodology and provides essential guidance on how to troubleshoot inefficient reactions. Experts in PCR describe design and optimization techniques, discuss the use of appropriate controls, explain the significance of standard curves, and explore the principles and strategies required for effective troubleshooting. The book highlights the importance of sample preparation and quality, primer design, controlling inhibitors, avoiding amplicon and environmental contamination, optimizing reagent quality and concentration, and modifying the thermal cycling protocol for optimal sensitivity and specificity. In addition, specific chapters discuss the history of PCR, the choice of instrumentation, the applications of PCR in metagenomics, high resolution melting analysis, the MIQE guidelines, and PCR at the microliter scale. The strategies, tips and advice contained in this concise volume will enable the scientist to optimize and effectively troubleshoot a wide range of techniques, including PCR, reverse transcriptase PCR, real-time PCR, and quantitative PCR. It will be an essential book for anyone using PCR technology.


Author: Alexander McLennan
Publisher: John Wiley & Sons
ISBN: 3527672095
Size: 20.92 MB
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"Molekularbiologie" ist das ma?geschneiderte Kurzlehrbuch f?r Studenten, die auf der Suche nach einer knappen Einf?hrung in dieses grundlegende Fachgebiet sind und die sich optimal f?r eine entsprechende Pr?fung vorbereiten wollen - auch dank der ?ber 70 Fragen und Antworten.

Molecular Biology

Author: Alexander McLennan
Publisher: Garland Science
ISBN: 0415684161
Size: 39.93 MB
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Rev. ed. of: Molecular biology / Phil Turner ... [et al.]. 3rd ed. New York, NY: Taylor & Francis, 2005.

Pcr Technology

Author: Henry A. Erlich
Publisher: Oxford University Press, USA
ISBN: 9780716770053
Size: 72.84 MB
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Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.

Pcr Technology

Author: Tania Nolan
Publisher: CRC Press
ISBN: 1439848130
Size: 25.72 MB
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PCR’s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology. Topics in this forward-thinking volume include: The purification and handling of PCR templates The effect of the manufacture and purification of the oligonucleotide on PCR behavior Optimum buffer composition Probe options The design and optimization of qPCR assays Issues surrounding the development and refinement of instrumentation Effective controls to protect against uncertainties due to reaction variability Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.


Author: Sir Norman Lockyer
Size: 55.38 MB
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Applications Of Polymerase Chain Reaction

Author: Sarika Garg
Publisher: Arcler Press
ISBN: 9781773611235
Size: 29.65 MB
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The polymerase chain reaction (PCR) is a widely used molecular biology technique. It was invented in 1983 by Kary Mullis, who was awarded a Nobel Prize in Chemistry in 1993 for his innovation. Since its introduction, the PCR technology has brought a true revolution in scientific research involving applied areas such as diagnosis and genetic improvements for plants and animals. PCR is a method of replicating deoxyribonucleic acid (DNA) by the selective amplification of a target region of a DNA molecule. Only few cycles of PCR can generate millions of copies of a particular DNA sequence quickly and precisely. Routinely, a PCR reaction consists of three distinct steps- denaturation, annealing and extension. In the first step of denaturation (at 95°C), double stranded DNA denatures into two single strands. In the annealing step (at 56°C), short complementary sequences of DNA known as primers, anneal to the single stranded target DNA. In the final step of extension, temperature is raised to around 72℃. DNA polymerase extends the primer to form a second complimentary strand of DNA. The elaboration of a PCR technique for amplification and for analyzing even very small amounts of DNA or a damaged DNA has generated wide applications in analysis of gene, diagnosis of several genetic diseases and detection of infectious agents like viruses, bacteria, fungi, protozoa, etc. Another beneficial application of PCR is in the generation of a genetic fingerprint. PCR is also used to monitor pathogens in the environment. PCR is an imperative technique for research in molecular biology such as in cloning procedure which has considerable potential in forensic science. PCR has numerous applications in various other fields such as agriculture sciences, personalized medicine, archeology, etc. This book gathers the recently published works related to PCR and its applications. The first chapter highlights the introduction to polymerase chain reaction. The second chapter features the application of reverse transcriptase-polymerase chain reaction (RT-PCR) in the rapid detection of pathogen in water samples. In chapter 3, discussion is on the PCR methods useful for accurate and rapid screening of the genetically modified organisms in foods. PCR applications in forensic science are described in chapter 4. Application of PCR for the diagnosis of ankylosing spondylitis is discussed in chapter 5. In chapters 6-12, use of PCR technique for the detection of pathogens and diagnosis of infectious diseases is described. PCR methods for the detection of Alzheimer's disease-related single nucleotide polymorphism and cancer related genes are illustrated in chapters 13 and 14, respectively. Chapter 15 discusses the PCR in exploring endodontic infections. Analysis of the archaeological skeletal remains using PCR is described in the last chapter of the book. I hope that this book will be advantageous for students, researchers, teachers, and industrial experts.

From Genes To Genomes

Author: Jeremy W. Dale
Publisher: John Wiley & Sons
ISBN: 1119954274
Size: 78.13 MB
Format: PDF
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The latest edition of this highly successful textbook introduces the key techniques and concepts involved in cloning genes and in studying their expression and variation. The new edition features: Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics. Clear, two-colour diagrams throughout. A dedicated website including all figures. Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.

Pharmaco Imaging In Drug And Biologics Development

Author: Brian R. Moyer
Publisher: Springer Science & Business Media
ISBN: 146148247X
Size: 58.98 MB
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The volume aim to be a comprehensive overview of the drug and biologic development process that is often called “the valley of death” (pre-IND through approval) where high costs of studies and high rates of product failure are part of the drug development landscape. Imaging tools can serve in this period by adding high value data, the images and the kinetic information they can provide, and cost-effective development alternative tools which potentially improve pivotal study designs. Imaging may identify safety issues early such as unwanted organ or tissue distributions, and then can serve advanced development with added certainty of a drug or biologic’s success to senior corporate management and investors. There are numerous textbooks, reference texts and treatises on medical imaging technologies, teaching tools on medical cases and physics books on the science of detector and computer interface systems. Rarely, in each of these are examples of medical imaging protocols and animal models of disease i.e. a text on methodology in drug development is currently unavailable.