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The Use Of Crispr Cas9 Zfns Talens In Generating Site Specific Genome Alterations

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Publisher: Academic Press
ISBN: 0128013346
Size: 55.74 MB
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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease

A Guide To Bioethics

Author: Emmanuel A. Kornyo
Publisher: CRC Press
ISBN: 1351803840
Size: 59.21 MB
Format: PDF, Kindle
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Solving intractable biotechnological questions of evolution, medicine, and genetics is now easier due to methods permitting the rapid analysis of molecular sequence data. These advances have exposed ethical and policy concerns. How would genomic information be used and by whom? Should individuals be able to make decisions regarding their own genomic data? How accurate are these genetic tests and how should they be regulated? These and other ethical conundrums are the subject of this book. Bioethicists, biomedical policy experts and lawyers, physicians, nursing and allied health students as well as science educators will find this book helpful and engaging in exploring the complexities of modern evolutionary, genetic and biomedical data.

Targeted Genome Editing Using Site Specific Nucleases

Author: Takashi Yamamoto
Publisher: Springer
ISBN: 4431552278
Size: 31.68 MB
Format: PDF, Mobi
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This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Phytonutritional Improvement Of Crops

Author: Noureddine Benkeblia
Publisher: John Wiley & Sons
ISBN: 1119079985
Size: 64.55 MB
Format: PDF
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An in-depth treatment of cutting-edge work being done internationally to develop new techniques in crop nutritional quality improvement Phytonutritional Improvement of Crops explores recent advances in biotechnological methods for the nutritional enrichment of food crops. Featuring contributions from an international group of experts in the field, it provides cutting-edge information on techniques of immense importance to academic, professional and commercial operations. World population is now estimated to be 7.5 billion people, with an annual growth rate of nearly 1.5%. Clearly, the need to enhance not only the quantity of food produced but its quality has never been greater, especially among less developed nations. Genetic manipulation offers the best prospect for achieving that goal. As many fruit crops provide proven health benefits, research efforts need to be focused on improving the nutritional qualities of fruits and vegetables through increased synthesis of lycopene and beta carotene, anthocyanins and some phenolics known to be strong antioxidants. Despite tremendous growth in the area occurring over the past several decades, the work has only just begun. This book represents an effort to address the urgent need to promote those efforts and to mobilise the tools of biotechnical and genetic engineering of the major food crops. Topics covered include: New applications of RNA-interference and virus induced gene silencing (VIGS) for nutritional genomics in crop plants Biotechnological techniques for enhancing carotenoid in crops and their implications for both human health and sustainable development Progress being made in the enrichment and metabolic profiling of diverse carotenoids in a range of fruit crops, including tomatoes, sweet potatoes and tropical fruits Biotechnologies for boosting the phytonutritional values of key crops, including grapes and sweet potatoes Recent progress in the development of transgenic rice engineered to massively accumulate flavonoids in-seed Phytonutritional Improvement of Crops is an important text/reference that belongs in all universities and research establishments where agriculture, horticulture, biological sciences, and food science and technology are studied, taught and applied.

Biotechnology Of Major Cereals

Author: Huw D Jones
Publisher: CABI
ISBN: 1780645198
Size: 61.27 MB
Format: PDF
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Biotechnology of Major Cereals will focus on the recent advances and future prospects in cereal biotechnology. The first part of the book will cover the world’s major cereals and focus on new developments and trends. The second part will be technology rather than species-led, detailing fundamental developments in technologies and significant target traits.

Eingriff In Die Evolution

Author: Jennifer A. Doudna
Publisher: Springer
ISBN: 9783662574447
Size: 20.70 MB
Format: PDF, ePub
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Die amerikanische Molekularbiologin Jennifer Doudna hat eine gentechnische Methode mitentwickelt, die von der New York Times als „eine der gewaltigsten Entdeckungen in der Biologie“ charakterisiert worden ist: die CRISPR-Technologie. In diesem Buch schildert sie, welches Potenzial diese Technik zur Veränderung alles Lebendigen birgt, und warnt zugleich vor den möglichen Gefahren ihrer Anwendung. Denn wir können mit der CRISPR-Technologie die genetische Information, die sämtliche Lebewesen steuert, erstaunlich einfach und überaus präzise verändern. Was einst eine Zukunftsvision der genetischen Manipulation war, wird auf einmal krasse Realität: Plötzlich sind wir in der Lage, Erbkrankheiten zu heilen, Leiden zu lindern und neue Nahrungs- und Energiequellen zu erschließen – aber auch jede Spezies einschließlich des Menschen zu unseren eigenen Zwecken umzugestalten. Doudna präsentiert hier zusammen mit ihrem Forscherkollegen Samuel Sternberg den maßgeblichen Bericht über die Entdeckung von CRISPR; sie erklärt, wie die geradezu wundersame Erfindung funktioniert und welche Möglichkeiten sie eröffnet. Aber ihr Buch fordert auch zum Nachdenken auf: Was bedeutet die neu gewonnene Macht? Wie können wir uns ihres beispiellosen Nutzens erfreuen und gleichzeitig ihren ebenso beispiellosen Gefahren aus dem Weg gehen? Antworten auf solche Fragen, so Doudna, betreffen uns alle. Wir müssen es schaffen, gemeinsam zu überlegen und zu handeln. Auf dem Spiel steht nichts Geringeres als die Zukunft der Menschheit – und aller Lebewesen auf der Erde. Dieses Buch ist ein unentbehrlicher Leitfaden für den Weg, der vor uns liegt.

Molekulare Humangenetik

Author: Tom Strachan
Publisher: Spektrum Akademischer Verlag
ISBN: 9783827414939
Size: 56.58 MB
Format: PDF
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Die vorliegende 3. Auflage der Molekularen Humangenetik ist völlig neu überarbeitet - unter Berücksichtigung der Entdeckungen, die im Zuge und in der Folge des Human Genome Project gemacht wurden. Die einführenden Kapitel (Teil I) beschreiben die Grundlagen wie DNA-Struktur und -Funktion, Chromosomen, Zellen und Entwicklung, Stammbaumanalysen und grundlegende Techniken im Labor. In Teil II werden die verschiedenen Genomsequenzierungsprojekte und die dadurch ermöglichten Einblicke in Organisation, Expression, Variabilität und Evolution des menschlichen Genoms gezeigt. Die Kartierung, Identifizierung und Diagnose der Ursachen von mendelnden und komplexen Krankheiten sowie Krebs ist Schwerpunkt von Teil III. Der letzte Teil gibt Ausblicke auf die funktionelle Genomik und Bioinformatik, auf Tiermodelle und Therapien. Das Buch soll eine Brücke bilden zwischen den grundlegenden Lehrbüchern und der Forschungsliteratur, sodass auch Interessierte mit relativ wenig Hintergrundwissen zum Thema die neuesten Forschungsergebnisse lesen und beurteilen können.

Entwicklung Der Gentherapie

Author: Senatskommission für Grundsatzfragen der Genforschung
Publisher: John Wiley & Sons
ISBN: 3527610537
Size: 66.28 MB
Format: PDF
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Seit die DFG im Jahre 1995 eine erste Stellungnahme zum Thema Gentherapie veröffentlichte, hat sich dieses Forschungsfeld ernorm weiterentwickelt. Diese neue Stellungnahme zeigt nun die aktuellen Themen und Perspektiven der gentherapeutischen Forschung auf.