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Thin Cell Layer Culture System Regeneration And Transformation Applications

Author: Duong Tan Nhut
Publisher: Springer Science & Business Media
ISBN: 9401735220
Size: 31.98 MB
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Scientists within the field of plant biotechnology are in a constant search for techniques that can, in the simplest manner possible, answer the genetic and biochemical questions that underlie developmental processes. Thin Cell Layer Culture System not only takes an in-depth look at a technique that has had so much success in attempting, through various practical models and systems, to answer these issues, but also represents a celebration of almost 30 years of research that has covered a massive scope of plant species and areas of study. The initial studies conducted on tobacco thin cell layers (TCLs) - proving that organogenesis can be strictly controlled in vitro - allowed plant research to benefit from this finding, expanding this knowledge in a practical and applied manner into the biotechnological fields of tissue culture and micropropagation, cell and organ genetics and biochemistry. The chapters in this book tell the enigmatic tale of TCLs. An historical perspective opens the scene for an inquiry into the possible cellular, biochemical and genetic processes that allow for the controlled development of a TCL into any organ type. The success of the system is further demonstrated in both monocotyledonous and dicotyledonous species, covering successful organogenesis and in vitro flowering in species within ornamental, leguminous and wood crops, cereals and grasses. Methodologies are outlined in detail, as is the rationale behind the TCL-organogenesis-developmental sequel. The TCL method, shown to be superior to many conventional micropropagation systems, has also shown to be vital in the recovery of transgenic plants. This book is an essential part of every plant, cell and developmental biologist, geneticist and tissue culturalist's shelf as it addresses the primary issue of any plant: the cell, the tissue, and their subsequent development into a highly organized system.

Agrobacterium Protocols Volume 2

Author: Kan Wang
Publisher: Springer Science & Business Media
ISBN: 1597451312
Size: 43.18 MB
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The second edition of Agrobacterium Protocols contains 80 chapters (two volumes) divided into 14 parts. In Volume II, there are a total of eight parts. Volume II contains seven of the eleven parts (Part VII to XIII) of plant protocols. Part XIV (Non-plants) contains six chapters with protocols for introducing DNA into non-plant species such as bacteria, fungi, algae, and mammalian cells. The description of this unique capacity of Agrobacterium is a new addition to this edition. This book provides a bench-top manual for tested protocols involving Agrobacterium-mediated transformation.

Plant Cell Culture

Author: Michael R. Davey
Publisher: John Wiley & Sons
ISBN: 9780470686515
Size: 52.32 MB
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The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Plant Biotechnology In Ornamental Horticulture

Author: Yi Li
Publisher: CRC Press
ISBN: 148229804X
Size: 68.43 MB
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Find out how biotechnology can produce more nutritious fruits and vegetables, more colorful flowers, and grass that needs less water—and mowing! Plant Biotechnology in Ornamental Horticulture presents an in-depth overview of the key scientific and technical advances, issues, and challenges in one of the fastest growing segments of the agriculture industry. This comprehensive book covers 19 different topics related to the use of transgenic plant technology to improve ornamental plants, ranging from metabolic engineering of flower color and scent to improving cold, drought, and disease tolerance in horticultural and ornamental crops to the economics of horticultural biotechnology. Horticulture provides color and flavor to the foods we eat and variety to the products we use, and helps us sustain a healthy environment. Plant Biotechnology in Ornamental Horticulture examines the importance of biotechnology in cultivating garden crops-including fruits, vegetables, flowers, and ornamentals such as plants used for landscaping-by reducing pesticide use, reducing soil erosion, and developing plants with improved nutrition. Leading educators and horticultural professionals address important current and future topics, including micropropagation and regeneration, the use of molecular techniques for genetic improvement, molecular-assisted breeding, abiotic stress, the development of disease resistance, protection from insects, herbicide tolerance, controlled flowering, modifying color and fragrance, plant architecture, and senescence. Plant Biotechnology in Ornamental Horticulture examines: * ornamental plant transformation * molecular phylogeny * drought response and drought tolerance engineering * transgenic approaches to viral, bacterial, and fungal disease resistance * vegetable propagation by cuttings * the promotion of flowering * molecular aspects of leaf morphogenesis * transgenic manipulation * controlling invasive plants * plant hormones, including ethylene, gibberellins (GAs), auxin, cytokinin, and abscisic acid (ABA) * and much more Plant Biotechnology in Ornamental Horticulture is essential reading for plant breeders, physiologists, agronomists, molecular biologists, cropping system specialists, as well as for educators and students involved in horticulture.

Journey Of A Single Cell To A Plant

Author: Susan J. Murch
Publisher: Science Pub Inc
ISBN: 9781578083527
Size: 15.93 MB
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In plants, the ability to regenerate identical individuals from single cells is the basis for modern agriculture. These scientific advancements have given us virus-free stocks, novel germplasms, clonal propagation systems, and the commercial introduction of difficult to propagate plant species. The timescale for breeding programs was dramatically reduced as plant tissue culture technologies were developed to shorten the time between generations and reduce the number of generations required for a line to be developed. Without the capacity to regenerate plants, it would not have been possible for plant biotechnology and genetic engineering to have advanced this far. This book contains detailed reviews by the leading scientists who made these discoveries.

Books In Print

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Books in print is the major source of information on books currently published and in print in the United States. The database provides the record of forthcoming books, books in-print, and books out-of-print.

Expanding The Production And Use Of Cool Season Food Legumes

Author: Frederick Joseph Muehlbauer
Publisher: Kluwer Academic Pub
ISBN: 9780792325352
Size: 74.64 MB
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The Second International Food Legume Research Conference (IFLRC-II) was held from 12--16 April 1992 in Cairo, Egypt. Five cool season food legume crops were included in the deliberations: pea (Pisum sativum), lentil (Lens culinaris), faba bean (Vicia faba), chickpea (Cicer arietinum), and grasspea (Lathyrus sativus). The principal objectives of IFLRC-II were to review and assess recent results from national and international research programs on cool season food legumes and to develop strategies for increasing the productivity, improving the quality and extending the use of these crops in different farming systems. Topics in both basic and applied research were addressed and multidisciplinary research efforts were emphasized. The papers published here are organized according to subject areas: Processing and Animal Feeds; Climate Change and Biotic and Abiotic Stresses; Host Plant Resistance to Manage Biotic Stresses; Policy Incentives; Breeding Methods and Selection Indices; Infrastructural Support; Cool Season Food Legume Breeding; Management to Control Biotic and Abiotic Stresses; Biotechnology and Gene Mapping; Crop Physiology and Productivity; and Farmers' Constraints and On-Farm Research. Sessions devoted to Regional Discussion Groups and to the Continuation of the IFLRC Concept were also included. A Conference Summary is presented by Professor Eric Roberts.

Plant Tissue Culture

Author: Sant Saran Bhojwani
Publisher: Elsevier Science
ISBN: 9780444888839
Size: 47.82 MB
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During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly. This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.