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Protein Purification

Author: Jan-Christer Janson
Publisher: John Wiley & Sons
ISBN: 1118002199
Size: 80.56 MB
Format: PDF
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The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography

Food Analysis

Author: S. Suzanne Nielsen
Publisher: Springer
ISBN: 3319457764
Size: 33.47 MB
Format: PDF
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This fifth edition provides information on techniques needed to analyze foods for chemical and physical properties. The book is ideal for undergraduate courses in food analysis and is also an invaluable reference to professionals in the food industry. General information chapters on regulations, labeling, sampling, and data handling provide background information for chapters on specific methods to determine chemical composition and characteristics, physical properties, and objectionable matter and constituents. Methods of analysis covered include information on the basic principles, advantages, limitations, and applications. Sections on spectroscopy and chromatography along with chapters on techniques such as immunoassays, thermal analysis, and microscopy from the perspective of their use in food analysis have been expanded. Instructors who adopt the textbook can contact the editor for access to a website with related teaching materials.

Industrial Biotechnology

Author: Christoph Wittmann
Publisher: John Wiley & Sons
ISBN: 3527341811
Size: 28.73 MB
Format: PDF, ePub, Docs
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The latest volume in the Advanced Biotechnology series provides an overview of the main product classes and platform chemicals produced by biotechnological processes today, with applications in the food, healthcare and fine chemical industries. Alongside the production of drugs and flavors as well as amino acids, bio-based monomers and polymers and biofuels, basic insights are also given as to the biotechnological processes yielding such products and how large-scale production may be enabled and improved. Of interest to biotechnologists, bio and chemical engineers, as well as those working in the biotechnological, chemical, and food industries.

Chemoselective And Bioorthogonal Ligation Reactions

Author: W. Russ Algar
Publisher: John Wiley & Sons
ISBN: 3527683445
Size: 30.52 MB
Format: PDF, ePub
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This timely, one-stop reference is the first on an emerging and interdisciplinary topic. Covering both established and recently developed ligation chemistries, the book is divided into two didactic parts: a section that focuses on the details of bioorthogonal and chemoselective ligation reactions at the level of fundamental organic chemistry, and a section that focuses on applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and benefits of the ligation reactions. With chapters authored by outstanding scientists who range from trailblazers in the field to young and emerging leaders, this book on a highly interdisciplinary topic will be of great interest for biochemists, biologists, materials scientists, pharmaceutical chemists, organic chemists, and many others.

Purification Tools For Monoclonal Antibodies

Author: Pete Gagnon
Publisher: Validated Biosystems Inc
Size: 22.19 MB
Format: PDF, ePub, Mobi
View: 1959
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Purification Tools for Monoclonal is an essential book for professionals, educators and advanced students in the field of Biotechnology. It is based on experience gained from purification process development, scale-up, and manufacture of more than 250 monoclonal-based diagnostic and therapeutic products. Ten chapters provide in-depth coverage of major separation mechanisms, process strengths, weaknesses and method development; all fully integrated with the special performance, economic and validation requirements associated with monoclonals. Covered methods include precipitation with inorganic salts, polyethylene glycol, electrolyte depletion, caprylic acid, ethacridine, chromatographic purification by size exclusion, ion exchange, hydroxyapatite, hydrophobic interaction, immobilized metal affinity, hydrophilic interaction, euglobulin adsorption, thiophilic adsorption, protein A, protein G, lectin affinity, and more. 88 figures, 29 tables.

Physical Biochemistry

Author: David Sheehan
Publisher: Wiley
ISBN: 9780471986638
Size: 74.20 MB
Format: PDF, ePub, Docs
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The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. - A major update of this comprehensive, accessible introduction to physical biochemistry. - Includes two new chapters on proteomics and bioinformatics. - Introduces experimental approaches with a minimum of mathematics and numerous practical examples. - Provides a bibliography at the end of each chapter Written by an author with many years teaching and research experience, "Physical Biochemistry: Principles and Applications, Second Edition" will prove invaluable to students of biochemistry, biophysics, molecular and life sciences and food science.

Short Protocols In Protein Science

Author: John E. Coligan
Publisher: John Wiley & Sons Inc
Size: 74.58 MB
Format: PDF, ePub
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Short Protocols in Protein Science provides condensed descriptions of more than 500 protocols compiled from Current Protocols in Protein Science. Drawing from both the original "core" manual as well as the quarterly update service, this compendium includes all step-by-step descriptions of the principal methods covered in Current Protocols in Protein Science.

Short Protocols In Cell Biology

Author: Juan S. Bonifacino
Publisher: John Wiley & Sons Incorporated
Size: 78.60 MB
Format: PDF, ePub
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Providing condensed descriptions of more than 500 methods compiledfrom Current Protocols in Cell Biology, this text thoroughlyexplores cell biology in an easily accessible, hands-on format.Short Protocols in Cell Biology is an authoritative andindispensable guide for all life scientists and researchers who arelooking to improve their understanding of cell biology methods. Key Features: Designed to provide quick access to step-by-step instructionsfor the essential methods used in every major area of cellbiological research Contains methods from every aspect of cell biology?everythingneeded to study the basic structure and functions of cells at boththe molecular and cellular levels